摘要
目的观察生白术对慢传输型便秘(STC)大鼠酪氨酸激酶生长因子受体(c-kit mRNA)表达的影响。方法 Wistar雄性大鼠30只,随机分为正常对照组、模型组、生白术治疗组,每组10只。正常对照组不造模,模型组及生白术治疗组采用大黄酸粉悬液灌胃制备STC大鼠模型。造模成功后正常对照组和模型组给予生理盐水灌胃,生白术治疗组给予生白术水提物溶液,各组连续灌胃给药2周。活性炭末灌胃测定肠道传输功能,并通过RT-PCR技术测定c-kit mRNA的表达情况。结果模型组与生白术治疗组大鼠大便性状较正常对照组明显变硬,且活性炭末长度及推进率明显低于正常对照组,差异有统计学意义(P<0.05)。经生白术治疗后活性炭末长度及推进率较模型组增加(P<0.05),模型组活性炭末长度及推进率明显低于正常对照组,差异有统计学意义(P<0.05)。模型组大鼠小肠、结肠c-kit mRNA表达明显低于正常对照组,差异有统计学意义(P<0.05),而胃窦c-kit mRNA表达与正常对照组比较无明显差异(P>0.05)。生白术治疗组与模型组比较,结肠c-kit mRNA表达明显增加(P<0.05),而胃与小肠c-kit mRNA表达的变化则无明显差异(P>0.05)。结论大剂量生白术治疗便秘的机制,可能是通过促进结肠组织c-kit mRNA表达,进而修复结肠Cajal间质细胞(ICC),恢复胃肠慢波节律的起搏,使结肠收缩活性增强,蠕动增加,加速结肠运动,最终治疗便秘,这可能是其治疗STC的机制之一。
Objective To observe the effect of rhizoma atractylodis macrocephalae on c - kit mRNA expression in the rats of slow transit constipation(STC). Methods Thirty Wistar male rats were randomized into a normal control group,a model group and a herb(rhizoma atractylodis macrocephalae)group,10 rats in each one. The modeling was not required in the normal control group. In the model group and the herb group, STC model was prepared by lavage with rhein powder suspension. The physical saline was used for lavage af-ter the success of modeling in the model group and the herb group. Additionally,the extract solution of rhizo-ma atractylodis macrocephalae was used in the herb group. The lavage was given continuously for 2 weeks in each group. The lavage of activated carbon was used to determine the bowel transient function. RT - PCR was used to determine the expression of c - kit mRNA. Results The stool condition was normal in the model group and the herb group and was getting solid apparently as compared with the normal control group. The pushing distance and rate of activated carbon were lower apparently than the normal control group,indicating the significant difference(P ﹤ 0. 05). After treated with rhizoma atractylodis macrocephalae,the pushing dis-tance and rate of activated carbon were increased as compared with the model group(P ﹤ 0. 05). In the model group,the expression of c - kit mRNA in the small intestine and colon was lower apparently than that in the blank control group,indicating the significant difference(P ﹤ 0. 05)and the expression in gastric antrum was not difference apparently(P ﹥ 0. 05). In comparison of the herb group and the model group,the expression of c - kit mRNA in the colon was increased apparently(P ﹤ 0. 05)and the expression in the stomach and small intestine was not different apparently(P ﹥ 0. 05). Conclusion Rhizoma atractylodis macrocephalae of large dose treats STC probably through promoting c - kit mRNA expression in colon tissue to repair colon ICC,re-cover the pace - making of gastrointestinal slow - wave rhythm,enhance colon contractive activity and peri-stalsis and accelerate colon movement.
出处
《世界中西医结合杂志》
2016年第8期1094-1097,共4页
World Journal of Integrated Traditional and Western Medicine
基金
北京市自然科学基金项目(7122180)
