摘要
为研究干酪乳杆菌对发育期腹泻模型大鼠回肠黏膜结构及黏蛋白2(MUC2)含量的影响,利用苏木精-伊红(HE)染色法、过碘酸雪夫(PAS)染色法、蛋白免疫印迹(Western-blot)及RT-PCR方法,以36只4周龄SD雄性大鼠为试验对象,随机分成6组,每组6只。采用5%硫酸镁溶液灌胃构建腹泻模型,10~8 CFU/mL干酪乳杆菌(LcZhang)液灌胃进行预防治疗,生理盐水灌胃作为空白对照。结果表明:干酪乳杆菌预防(6 h)组回肠V/C值、杯状细胞数量、MUC2蛋白含量及MUC2 mRNA的表达量比腹泻(6 h)组分别增加了10.89%、9.28%、26.44%和66.67%(P=0.00,P<0.01,P<0.01,P<0.05);干酪乳杆菌预防(48 h)组回肠V/C值比腹泻(48 h)组显著增加了12.20%(P=0.00)。以上结果显示,饲用干酪乳杆菌进行预防能够显著提高腹泻大鼠回肠V/C值及杯状细胞数量,促进MUC2的合成,表明干酪乳杆菌可以有效维护4周龄大鼠肠道健康,减轻腹泻对大鼠肠道黏液屏障的损害,加强肠道免疫功能。
In order to study the effect of Lactobacillus casei on ileal mucosal structure and MUC2 content of rats with developmental diarrhea,36 male 4-weed-old Sprague-Dawley rats were randomly divided into 6 groups with 6 in each group.Hematoxylin-eosin staining,Periodic acid Schiff staining,Western blot and RT-PCR methods were adopted in this study.In the experiment,a diarrhea model was established by intragastric administration of 5%magnesium sulfate solution and 10~8 CFU/mL Lactobacillus casei Zhang(LcZhang)solution was intragastrically administered for prevention and treatment.Saline treatment only was administered as a control.The results showed that:The ileal V/C value,number of goblet cells,MUC2 content and expression of MUC2 mRNA in group of the Lactobacillus casei prevention group(6 h)were respectively increased by 10.89%,9.28%,26.44%,66.67%(P=0.00,P<0.01,P<0.01 and P<0.05),compared with the group of diarrhea group(6 h);The ileal V/C value of group in the Lactobacillus casei prevention group(48 h)was significantly increased by 12.20%(P=0.00),compared with the diarrhea group(48 h).The above results showed that the prevention of Lactobacillus casei significantly increased the ileal V/C value and the number of goblet cells in diarrhea rats,and promoted the synthesis of MUC2,indicating that Lactobacillus casei could effectively maintain the intestinal health of 4-week-old rats,reduce the damage of diarrhea on intestinal mucus barrier and strengthen intestinal immune function.
作者
王誉颖
汤林杰
李姣
王佳
闫雪
韩德平
滕可导
马云飞
WANG Yuying;TANG Linjie;LI Jiao;WANG Jia;YAN Xue;Han Deping;TENG Kedao;MA Yunfei(College of Veterinary Medicine,China Agricultural University,Beijing100193,China;State Key Laboratory of Direct-Fed Microbial Engineering,Beijing DaBeiNong Science and Technology Group Co.,Ltd.,Beijing100192,China)
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2019年第8期94-101,共8页
Journal of China Agricultural University
基金
北京大北农科技集团股份有限公司饲用微生物工程国家重点实验室开放课题(201205410511191)
国家自然科学基金资助项目(31772686,31502025)
作者简介
第一作者:王誉颖,硕士研究生,E-mail:wangyuying96@163.com;通讯作者:马云飞,副教授,主要从事动物组织胚胎学与神经生物学研究,E-mail:yunfeima@cau.edu.cn
