摘要
为研究干酪乳杆菌对断奶仔猪十二指肠黏膜组织结构和功能及发育的影响,采用组织化学和免疫组织化学方法,以168头14日龄断奶仔猪为试验对象,随机分成7组,每组4个重复。研究结果显示:1)干酪乳杆菌预防组(E组)、预防治疗组(F组)的肠绒毛高度分别比大肠杆菌K88攻毒组(D组)明显增高了11.6%(P<0.01)和11.2%(P<0.01)。2)E组、F组的绒毛高度与肠隐窝深度的比值(V/C)分别比D组显著增加了21.3%(P<0.01)和20.5%(P<0.01)。3)E组、F组的肌层厚度分别比D组增加了11.8%(P<0.05)和10.2%(P<0.05)。4)D组、E组、F组和直接治疗组(G组)各组间增殖细胞核抗原(PCNA)含量在统计学上差异不显著,但使用干酪乳杆菌预防和治疗的E组和F组的增殖细胞核抗原(PCNA)含量均高于K88攻毒的D组。以上结果表明,饲用干酪乳杆菌进行预防能够显著提高断奶仔猪小肠绒毛高度,明显增加V/C值、肌层厚度及PCNA含量,提示干酪乳杆菌能有效维护断奶仔猪肠道健康,减轻大肠杆菌对肠黏膜结构的损害,促进肠道的发育。
In order to study the effect of Lactobacillus casei on the development of the small intestine in weaned pigs, we explored the effects of Lactobacillus casei on the development and mucosal structure of the duodenum in 14-day-old weaned pigs by using histochemistry and immunohistochemistry. The results were as follows： 1） villus height of the prevented group （group E） and the prevented-and-treatment group （group F） was significant higher than Escherichia coil K88 infection group （group D） by 11.6% （P〈0.01） and 11.2% （P〈0.01） respectively. 2） V/C ratio of the group E and the group F was significant higher than the group D by 21.3% （P〈0.01） and 20.5% （P〈0.01） respectively.3） muscular thickness of the group E and the group F was thicker than the group D by 11.8% （P〈0.05） and 10.2% （P〈0.05） respectively. 4） there were no statistically significant differences in PCNA content between the group D,the group E,the group F and the direct treatment group （group G） ,but the PCNA content was higher in group E and group F than that of the group D. These results suggested that, prevention with Lactobacillus casei in food could obviously increase the villus height, V/C ratio, musculature thickness and PCNA content, indicating that Lactobacillus casei would effectively alleviate structural damage to the intestinal mucosa caused by Escherichia coil and promote healthy gut development in weaned pigs.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2015年第3期114-120,共7页
Journal of China Agricultural University
基金
国家973计划项目(2012CB723702)
北京大北农科技集团股份有限公司饲用微生物工程国家重点实验室开放课题(201205410511191)
国家国际科技合作专项项目(2011DFA32330)
关键词
仔猪
干酪乳杆菌
十二指肠
发育
组织学
piglets
Lactobacillus casei
duodenum
development
histology
作者简介
张魏伟，硕士研究生，E-mail：ps_zww@cau．edu．cn
马云飞，副教授，主要从事动物组织胚胎学与神经生物学研究，E-mail：yunfeima@call．edu．cn
