摘要
目的:YLT-322是本实验室合成的一种新型的苯并噻唑衍生物,已有文献证实其可以通过线粒体途径诱导肝癌细胞Hep G2凋亡,对多种癌细胞都有效果。本实验拟通过一系列生物实验来考察YLT-322对结直肠癌细胞的作用。方法:作者对多种癌细胞进行初筛,筛选出两种效果较好的结直肠癌细胞进行细胞毒性试验,平板克隆形成,Hoechst等实验,以证实YLT-322对结直肠癌有良好的抑制作用。结果:YLT-322实验组比对照组的细胞凋亡明显增多,且与剂量和作用时间成正比。说明YLT-322的确对结直肠癌有抑制作用。结论:YLT-322对结直肠癌细胞有诱导凋亡的作用,作为一个新型的小分子抑癌药物,具有很大的开发价值。
Objective: YLT-322 was a new benzothiazole derivatives synthesized in our laboratory. There were literature reported that it could induce apoptosis in Hep G2 via mitochondrial pathway. And it had effects on a variety of cancer cells. This experiment meant to investigate the effect of YLT-322 on colorectal cancer cells by a series of biological experiments. Method:Many kinds of cancer cells were screened. Two colorectal cancer cells were selected to carry out toxicity test, plate clone formation, Hoechst experiment to confirm that YLT322 had good inhibition effect on colorectal cancer. Result: Experimental group treated with YLT-322 significantly increased apoptotic cells compared with control group. And the effect of YLT-322 was proportional to the dose and duration time which confirmed the inhibition of YLT-322 on colorectal cancer. Conclusion: YLT-322 induce apoptosis in colorectal cancer cells. It has great value of development as a novel small molecule anti cancer drug.
出处
《中药与临床》
2015年第2期72-74,共3页
Pharmacy and Clinics of Chinese Materia Medica
