摘要
目的研究miR-133b在胃癌细胞、正常胃黏膜上皮永生化细胞、胃癌组织及相应癌旁组织中的表达,探讨miRNA对胃癌发生发展的调控作用。方法培养7种胃癌细胞株及正常胃黏膜上皮细胞,并收集56例胃癌患者癌组织及相应癌旁组织,提取细胞及组织中总的Small RNA,采用real-time PCR法检测miR-133b在胃癌细胞及组织中的表达,分析miR-133b的表达与细胞分化程度、临床病理特征的关系。结果 miR-133b在胃癌细胞及组织中均表达下调,差异具有统计学意义。miR-133b的低表达与细胞分化程度、TNM分期及有无淋巴结转移显著相关。结论 miR-133b在胃癌细胞及胃癌组织中的表达明显下调,可能作为抑癌基因参与胃癌的发生、发展。
Objective To investigate the expression of mi R-133b in human gastric cancer cell lines, gastric mucosal epithelial cells in vitro, gastric cancer tissues and matched adjacent non-tumor tissues, and to explore the mi RNA regulation role in the occurrence and development of gastric cancer. Methods We cultivated 7 gastric cancer cell lines and normal gastric mucosa epithelial cells, and collected 56 cases of gastric cancer and the corresponding para-carcinoma tissues, to extract the total Small RNA. After reverse transcription, real-time PCR was used to detect the expression of mi R-133b in gastric cancer cells and tissues, to analyze its association with clinicopathological features and cell differentiation. Results The expression level of mi R-133b was down-regulated in human gastric cancer cells and tissues, with statistic difference. The down-regulated miR-133b expression was associated with cell differentiation, lymph node metastasis and TNM stage in gastric cancer patients(P<0.05).Conclusion The expression of miR-133b is significantly decreased in gastric cancer cells and tissues. miR-133b may be involved in the tumorigenesis of gastric cancer as an anti-oncogene.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2015年第5期474-477,共4页
Cancer Research on Prevention and Treatment
