摘要
目的:研究鳄嘴花正丁醇提取物[Clinacanthus nutans(Burm.f.)Lindau n-butanol extracts,CN-N]对荷瘤小鼠的体内抑瘤作用及延长生存时间作用。方法:肝癌细胞接种至ICR小鼠右侧腋下,建立Heps小鼠肝癌模型,随机分成4组,对照组、环磷酰胺组、CN-N低剂量组(3 mg·kg-1·d-1)、CN-N高剂量组(10 mg·kg-1·d-1),每组10只,观察CN-N对荷瘤小鼠的抑瘤率、体质量、脾脏及胸腺指数、生存时间的影响。蛋白质印迹法检测增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达,TUNEL法检测细胞凋亡情况。结果:与对照组比较,CN-N低剂量组和高剂量组肿瘤质量明显降低(t分别为2.261,3.140,P均<0.05),荷瘤小鼠脾脏指数、胸腺指数差异无统计学意义(P均>0.05),PCNA表达均明显减少;CN-N高剂量组生命延长率为54.8%,较对照组明显延长荷瘤小鼠生存期(t=3.416,P=0.003 1)。结论:鳄嘴花正丁醇提取物具有良好的抗肿瘤作用,高剂量CN-N能提高荷瘤小鼠的生存期。
Objective:To investigate the anti-tumor activity and life extention effect of Clinacanthus nutans (Burm.f.) Lindau n-butanol extract (CN-N) on tumor loaded mice.Methods:Mice hepatoma carcinoma cells (Heps,grown in donor mice) were transplanted subcutaneously into armpit of the ICR mice to establish the tumor xenografts model.Tumor mice were randomized into four groups:control group,cytoxan group,CN-N low dose group (3 mg · kg-1 · d-1),CN-N high dose group (10 mg · kg-1 · d-1).Tumor inhibition rate,body weights,immune organs index and life-prolonging effect were recorded.Proliferation of tumor cells were detected by western blotting and apoptosis was examined by TUNEL assay.Results:Compared with the control group,CN-N low dose group and high dose group showed remarkable inhibition of tumor weight (t =2.261,3.140,both P ＜0.05) ;no significant differences on spleen and thymus index of tumor-bearing mice (both P ＞ 0.05).PCNA expression levels were greatly reduced when treated with low or high concentration of CN-N.Compared with the control group,CN-N high dose group significantly extended the survival time(t =3.416,P =0.003 1).Conclusion:CN-N exhibited good antitumor activity and significantly prolonged the survival time of the tumor-bearing mice.
出处
《江苏大学学报（医学版）》
CAS
2014年第3期211-215,共5页
Journal of Jiangsu University：Medicine Edition
关键词
鳄嘴花
正丁醇提取物
抗肿瘤
细胞凋亡
Clinacanthus nutans
n-butanol extracts
antitumor
apoptosis
