摘要
以猪霍乱沙门氏菌作为包被抗原 ,利用沙门氏菌O7单克隆抗体酶结合物建立了竞争ELISA方法检测猪霍乱沙门氏菌抗体。经过研究确定抗原包被浓度为 2×1 0 8CFU/ml,血清检测稀释度为 1∶1 6,酶标抗体浓度为 1∶70 0 ,包被液为碳酸盐缓冲液 ( 0 .0 5MpH9.6)。应用单抗竞争ELISA和平板凝集试验 (PAT)同时对 5 0 6份血清进行猪霍乱抗体检测 ,PAT的检出率为 3 .60 % ,ELISA检出率为 5 .75 % ,两者符合率达 96.4%。试验结果表明 ,ELISA方法敏感性高 ,特异性强 ,稳定性和重复性好 ,操作简便。本方法的建立在猪群沙门氏菌感染的检疫、实验室诊断的标准化及流行病学调查方面具有应用价值。
Optimum conditions of cELISA were developed as follows;2×10 8CFU/ml of killed Samlmonella choleraesuis in carbonate sodium buffer(0.05M,pH9.6)used to coat ELISA plated,1:700 dilution of HRP-labelled MAb O 7 as detecting regent,and 1:16 of sera as detecting samples.A total of 506 sera wre detected in parallel by both the cELISA and plate agglutination test(PAT).5.75% of them were detected positively as piglet's paratyphoid,while 3.60% of them wrer positive by PAT.These results showed that the cELISA has the advantage of higher sensitivity,specificity,reproducibility and stability.It would be very useful in diagnosis,surveillance of piglet's paratyphoid.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2003年第1期65-68,76,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
高校优秀青年教师教学科研奖励计划资助
扬州大学生物科学与技术学院青年教师科研启动基金资助
