摘要
为了明确繁殖养殖一体模式和繁殖养殖分离模式下春季克氏原螯虾养殖系统细菌群落变化及其影响因子,本研究利用16S rRNA基因测序对成熟期克氏原螯虾养殖系统水体、底泥和虾肠道细菌群落组成和多样性进行分析,阐明两种养殖模式下养殖系统细菌群落特征及其与水质因子关系的差异。结果表明:繁殖养殖一体模式水体为中度富营养化水平,繁殖养殖分离模式水体为中营养水平。水体、底泥和虾肠道细菌群落多样性两种模式间无显著差异,群落组成有显著差异。与繁殖养殖一体模式相比,繁殖养殖分离模式显著提高了水体变形菌门细菌和底泥放线菌门细菌的相对丰度,降低了水体蓝细菌门细菌和放线菌门细菌的相对丰度,减少了肠道中潜在致病菌鲁氏不动杆菌的相对丰度,降低了克氏原螯虾肠道遭受致病菌感染的风险。总氮(TN)含量、磷酸盐(PO_(3)^(4-)-P)含量,叶绿素a含量和高锰酸盐指数(COD_(Mn))是繁殖养殖一体模式水体、肠道菌群组成的主要影响因子;透明度,水温和水体溶解氧(DO)含量是繁殖养殖分离模式水体、肠道菌群组成的主要影响因子。本研究结果为克氏原螯虾养殖水质调控、疾病预防和稻虾种养绿色可持续发展提供了理论支撑。
To clarify the changes in bacterial communities and their influencing factors in the spring rice-crayfish co-culture system(RCCS)under integrated and isolated breeding modes,16S rRNA gene sequencing was used to investigate the composition and diversity of bacterial communities in the pond water,sediment and gut of crayfish at mature stage.The bacterial community characteristics and their relationships with water quality factors were compared and studied under the separation and integration of breeding and rearing modes.The results showed that the water under the integrated mode reached a moderately eutrophic level,while the water under the separated mode maintained a mesotrophic state.Bacterial community diversity in water,sediment and crayfish gut samples showed no significant differences between the two modes,but community composition exhibited significant divergence.Compared to the integrated mode,the separated mode significantly increased the relative abundance of Proteobacteria in water and Actinobacteria in sediment and decreased the relative abundance of Cyanobacteria and Actinobacteria in water,reduced the relative abundance of potential pathogen Acinetobacter lwoffii in crayfish gut,and lowered the risk of pathogenic bacterial infection in crayfish gut.In the integrated breeding-cultivation mode,total nitrogen(TN)content,orthophosphate(PO_(3)^(4-)-P)content,chlorophyll a content,and permanganate index(COD_(Mn))were identified as the primary factors influencing bacterial community composition in both water and crayfish gut microbiota.In the separated breeding-cultivation mode,water transparency,temperature,and dissolved oxygen(DO)content were identified as the primary factors influencing bacterial community composition in aquatic environments and intestinal microbiota.This study provides a theoretical foundation for optimizing water quality management,preventing diseases,and advancing green sustainable development in rice-crayfish co-culture systems.
作者
覃宝利
韩光明
吴雷明
马林杰
徐荣
杨婷
张诚信
毕建花
王守红
朱凌宇
张家宏
寇祥明
QIN Baoli;HAN Guangming;WU Leiming;MA Linjie;XU Rong;YANG Ting;ZHANG Chengxin;BI Jianhua;WANG Shouhong;ZHU Lingyu;ZHANG Jiahong;KOU Xiangming(Institute of Agricultural Sciences of the Lixiahe District in Jiangsu Province,Yangzhou 225007,China)
出处
《江苏农业学报》
北大核心
2025年第7期1388-1397,共10页
Jiangsu Journal of Agricultural Sciences
基金
江苏省科技计划项目(BN2023054)
江苏现代农业(克氏原螯虾)产业技术体系项目[JATS(2023)241]
扬州市科技计划项目(YZ2023046、YZ2023244)
江苏里下河地区农科所克氏原螯虾综合种养关键技术创新与应用项目[SJ(22)205]。
关键词
克氏原螯虾
养殖模式
细菌群落
水质因子
Procambarus clarkii
cultivation modes
bacterial community
water quality factor
作者简介
覃宝利(1986-),女,河南鹤壁人,硕士,助理研究员,主要从事稻渔综合种养技术研究。(E-mail)20141602@jaas.ac.cn;通讯作者:寇祥明,(E-mail)kouxiangming@163.com。
