摘要
耐盐西瓜种质资源匮乏,限制了其在盐碱地的规模化生产。以12份西瓜种质为试材,研究了不同浓度盐处理(0、100、150、200 mmol·L^(-1)NaCl)对其萌发的影响,分别测定萌发率、发芽指数、发芽势等指标,利用隶属函数分析和聚类分析等方法对12份西瓜种质材料进行耐盐性综合评价。结果表明,与对照(0 mmol·L^(-1)NaCl)相比,不同浓度的盐处理降低了大部分西瓜种子的发芽势、发芽指数、芽长、活力指数等,盐害指数反之;12份西瓜种质在萌发期的耐盐性呈现明显差异。通过隶属函数及聚类分析,确定W62、E125、E62、B24等4份种质为耐盐材料;B12、C84、C21、A79、D12等5份种质为中等耐盐材料;C5、D47、B56等3份种质为盐敏感材料。研究结果为西瓜耐盐基因挖掘及耐盐品种选育提供了材料。
The scarcity of salt-tolerant watermelon germplasm resources limits its large-scale production in saline soils.Using 12 watermelon germplasm as test materials,the effects of different concentrations of salt treatments(0,100,150 and 200 mmol·L^(-1) NaCl)on their germination were studied.The germination rate,germination index,and germination potential were measured,and the salt tolerance of the 12 watermelon germplasm were comprehensively evaluated using methods such as subordinate function analysis and cluster analysis.The results showed that compared with CK(0 mmol·L^(-1) NaCl),salt treatments at different concentrations reduced the germination potential,germination index,shoot length,and vigor index in most watermelon germplasm,while the salt injury index was the opposite.The salt tolerance of the 12 watermelon germplasms showed significant difference during the germination stage.By using the subordinate function and cluster analysis,the germplasm resources were classified as follows:W62,E125,E62 and B24 as salt-tolerant,B12,C84,C21,A79 and D12 as moderately salt-tolerant,C5,D47 and B56 as salt-sensitive.The research results provide materials for the exploration of salt-tolerant genes and the breeding of salt-tolerant varieties in watermelon.
作者
王明笛
张妍楚
李兴远
王彬权
黄战
刘金宝
张云玲
由守昌
张锐敏
WANG Mingdi;ZHANG Yanchu;LI Xingyuan;WANG Binquan;HUANG Zhan;LIU Jinbao;ZHANG Yunling;YOU Shouchang;ZHANG Ruimin(College of Horticulture,Shandong Agricultural University,Taian 271018,Shandong,China;UNELL Biotechnology Co.,Ltd.,Weifang 261101,Shandong,China;Weifang Guopai Agricultural Technology Co.,Ltd.,Weifang 261101,Shandong,China)
出处
《中国瓜菜》
北大核心
2025年第7期62-71,共10页
China Cucurbits And Vegetables
基金
宁波市公益性科技计划项目(2024S114)
山东省科技型中小企业创新能力提升工程(2022TSGC2338)
山东省重点扶持区域引进急需紧缺人才项目(2024-94)
山东省重点研发计划(良种工程)(2022LZGCQY00601)
山东省蔬菜产业技术体系(SDAIT-05)。
关键词
西瓜
耐盐
萌发期
聚类分析
种质筛选
Watermelon
Salt tolerance
Germination stage
Cluster analysis
Germplasm screening
作者简介
王明笛,女,在读本科生,主要从事蔬菜育种工作。E-mail:sdtamd@163.com;通信作者:张锐敏,女,副教授,主要从事西瓜遗传育种工作。E-mail:zrm0923@sdau.edu.cn。
