摘要
为了开发能够精准鉴别云南黄连不同基因型的分子标记以便为种质真实性鉴定提供依据,利用5个云南黄连群体的简化基因组重测序数据,筛选获得34891个有变异的单核苷酸多态性(Single Nucleotide Polymorphism,SNP)标记。基于所有的SNP位点信息构建的系统发育树表明:来自福贡县鹿马登乡的群体2和福贡县石月亮乡的群体3之间的亲缘关系较近;来自贡山县独龙江乡的群体1与来自泸水市老窝镇的群体5以及福贡县上帕镇的群体4的亲缘关系均较远。筛选出10个核心SNP标记,成功构建了5个云南黄连群体的分子身份编码和二维码,可用于鉴别不同基因型的云南黄连。
To develop molecular markers capable of accurately discriminating among different genotypes of Coptis teeta and thereby provide a basis for germplasm authenticity verification,using the reduced-representation genome resequencing data from five populations of Coptis teeta,and 34891 Single Nucleotide Polymorphism(SNP)markers with variations were screened and obtained.The phylogenetic tree constructed based on all SNP locus information indicated that Population 2 from Lumadeng Township,Fugong County,and Population 3 from Shiyueliang Township,Fugong County was relatively close.Population 1 from Dulongjiang Township,Gongshan County,exhibited a relatively distant genetic relationship with both Population 5 from Laowo Town,Lushui City,and Population 4 from Shangpa Town,Fugong County.Ten core SNP markers were selected,and molecular identity codes along with QR codes were successfully established for the five Coptis teeta populations,which can be used to identify different genotypes of this species.
作者
李月仙
姜太玲
严炜
沈绍斌
肖明昆
白丽娜
段春芳
罗鑫
车彬
张林辉
LI Yuexian;JIANG Tailing;YAN Wei;SHEN Shaobin;XIAO Mingkun;BAI Lina;DUAN Chunfang;LUO Xin;CHE Bin;ZHANG Linhui(Tropical and Subtropical Economic Crops Institute,Yunnan Academy of Agricultural Sciences,Baoshan Yunnan 678000,China)
出处
《西南大学学报(自然科学版)》
北大核心
2025年第5期103-112,共10页
Journal of Southwest University(Natural Science Edition)
基金
云南省重点研发计划项目(201203AC100003-1-6)。
关键词
云南黄连
简化基因组重测序
单核苷酸多态性
系统发育树
分子身份证
Coptis teeta Wall.
simplified genome resequencing
single nucleotide polymorphism
phylogenetic tree
molecular identity
作者简介
李月仙,硕士研究生,主要从事中药材资源与育种研究;通信作者:张林辉,研究员。
