摘要
目的分析利川黄连中小檗碱含量,以确定利川黄连的最佳栽培方案。方法利用高效液相色谱(HPLC)方法测定不同海拔高度,不同株龄及不同叶片形态的利川黄连小檗碱含量。结果黄连根茎中的小檗碱含量不受株龄的影响;低海拔地区比较适宜黄连小檗碱的合成;冷杉林遮光不影响小檗碱的合成;大花叶和小花叶品种相对较有光叶和无光叶植株根茎中小檗碱含量高,但无统计学差异。结论小檗碱的合成与遮荫条件密切相关,如果在第2～3年降低遮荫率或拆除遮荫棚以促进黄连根茎快速肥大,在采收前1年再通过遮光处理促进小檗碱的合成,可能会获得小檗碱含量更高的优质黄连。
Objective To determine the contents of berberine in Coptis chinensis of Lichuan and establish its best cultivation scheme. Method We used high performance liquid chromatographyHPLCto measure the contents of berberine at different altitude growth age and leaves. Results Analytic data showed that the growth age and firry woods shading did not affect the contents of berberine in rhizome of Coptis chinensis. Low altitude was more suitable for Coptis chinensis to synthesize berberine. The contents of berberine in rhizome of Coptis chinensis with floral leaf were higher than those with lusterless and lus-trous leaves but no significant difference. Conclusion The synthesis of berberine is closely correlated with shading conditions. It is recommended that the shading ratio should be reduced or the sheds removed in the middle growth age2-3 years and then the herb should be reshaped at the last year to enhance the synthesis of berberine so as to obtain high-quality Coptis chinensis in the harvest.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2004年第6期618-621,共4页
Acta Academiae Medicinae Sinicae
关键词
利川
黄连
小檗碱
Lichuan Coptis chinensis berberine
