摘要
CD146是免疫球蛋白超家族成员,在脂肪组织中高表达,参与调节脂肪细胞分化、细胞迁移、免疫应答。为研究奶牛泌乳过程中CD146是否参与乙酸钠诱导的乳脂合成过程,采用荧光定量PCR、免疫印迹方法检测乙酸钠处理的奶牛乳腺上皮细胞中CD146的表达。结果表明:乙酸钠能够显著促进CD146 mRNA和蛋白水平表达。试验成功构建CD146干扰载体,通过瞬时转染方法转染乙酸钠诱导的奶牛乳腺上皮细胞,CD146干扰载体转染后抑制奶牛乳腺上皮细胞由乙酸钠诱导的CD146及乳脂合成相关基因表达,同时,细胞内甘油三酯合成下降。
CD146,a member of the immunoglobulin superfamily,is highly expressed in adipose tissue and is involved in the regulation of adipocyte differentiation,cell migration,and immune response.In order to investigate whether CD146 is involved in sodium acetate-induced milk fat synthesis during lactation in dairy cows,in this study,we firstly detected the expression of CD146 in mammary epithelial cells of dairy cows treated with sodium acetate by using fluorescence quantitative PCR and immunoblotting,and the results showed that sodium acetate could significantly promote the expression of CD146 at both mRNA and protein levels.Subsequently,the experiment successfully constructed CD146 interference vector and transfected dairy cow mammary epithelial cells induced by sodium acetate by transient transfection,and the results showed that the CD146 interfering vector inhibited the expression of CD146 and genes related to milk fat synthesis induced by sodium acetate in dairy cow mammary epithelial cells,and decreased intracellular and triglyceride content.
作者
徐自如
葛慧聚
刘佳琪
随风贤
刘美妍
杨洋
侯晓明
林叶
XU Ziru;GE Huiju;LIU Jiaqi;SUI Fengxian;LIU Meiyan;YANG Yang;HOU Xiaoming;LIN Ye(Heilongjiang Provincial Key Laboratory of Cellular and Genetic Engineering,Northeast Agricultural University,Harbin 150030,China)
出处
《东北农业大学学报》
北大核心
2025年第1期61-69,共9页
Journal of Northeast Agricultural University
基金
国家自然科学基金面上项目(31771453)。
关键词
CD146
奶牛
乳腺上皮细胞
乙酸钠
乳脂合成
CD146
dairy cow
mammary epithelial cells
sodium acetate
milk fat synthesis
作者简介
第一作者:徐自如(1998-),女,硕士,研究方向为乳腺发育及泌乳生物学。E-mail:2547798662@qq.com;通信作者:林叶,教授,博士生导师,研究方向为乳腺发育及泌乳生物学。E-mail:linlu516@163.com。
