摘要
目的基于超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF-MS/MS)分析鉴定紫地榆中的化学成分。方法采用ACQUITY UPLC-BEH C18(2.1 mm×100 mm,1.7μm)色谱柱,以乙腈-0.1%甲酸溶液为流动相,进行梯度洗脱;质谱条件:采用ESI电喷雾离子源,进行正、负离子全扫描。结果通过对质谱数据分析并参考相关文献,结合对照品比对结果,共鉴定出67个化学成分,包含鞣质类21个、有机酸类20个、黄酮及其苷类15个、萜类11个。结论本研究建立的方法快速、准确,可为紫地榆的质量控制及物质基础研究提供参考依据。
OBJECTIVE To identify the chemical constituents in Geranium Strictipes Knuth based on ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS).METHODS Using ACQUITY UPLC-BEH C18(2.1 mm×100 mm,1.7μm)column,using acetonitrile-0.1%formic acid solution as mobile phase for gradient elution.Mass spectrometry conditions:using ESI electron Spray ion source for full scan of positive and negative ions.RESULTS By analyzing the mass spectrometry data and referring to relevant literature,combined with the comparison results of reference substances,a total of 67 chemical components were identified,including 21 tannins,20 organic acids,15 flavonoids and their glycosides,and 11 terpenes.CONCLUSION The rapid and accurate establishment of this study can provide a reference for the control research and material basis research of Geranium Strictipes Knuth.
作者
郗仲玟
吴玲芳
冯玉
田伟
牛丽颖
田宇柔
XI Zhongwen;WU Lingfang;FENG Yu;TIAN Wei;NIU Liying;TIAN Yurou(Hebei University of Traditional Chinese Medicine,Shijiazhuang 050091,China;The First Affiliated Hospital of Hebei University of Chinese Medicine,Shijiazhuang 050011,China;Quality Evaluation&Standardization Hebei Province Engineering Research Center of Traditional Chinese Medicine,Shijiazhuang 050091,China;Hebei Traditional Chinese Medicine Formula Granule Technology Innovation Center,Shijiazhuang 050091,China)
出处
《中国现代应用药学》
北大核心
2025年第3期368-376,共9页
Chinese Journal of Modern Applied Pharmacy
基金
河北省重点研发计划项目(23372502D)
河北省省属高校基本科研业务费(TDZR2024004)。
作者简介
郗仲玟,女,硕士,E-mail:17798001673@163.com;通信作者:牛丽颖,女,硕士,教授,博导,E-mail:niuliyingyy@hebcm.edu.cn;通信作者:田宇柔,女,硕士,高级实验师,E-mail:tianyurou@hebcm.edu.cn。
