摘要
玉米赤霉烯酮(ZEN)是一种具有类雌激素效应的霉菌毒素,在饲料和饲料原料中经常检出,严重威胁畜禽健康。试验研究了米曲霉HAU491降解ZEN的特性,然后将其作为菌种用于豆粕发酵,实现对豆粕中ZEN的脱除。研究结果表明,米曲霉HAU491可在3 h内将反应体系中初始浓度为2.5μg/mL的ZEN降解98.1%;其降解ZEN的最适pH范围为5.0~7.0;在22~52℃温度范围内,米曲霉HAU491对ZEN的2 h降解率维持在90%以上。有效降解成分分析发现米曲霉HAU491降解ZEN的活性物质主要存在于菌体中,菌体经过100℃水浴10 min、EDTA、蛋白酶K和十二烷基硫酸钠(SDS)处理后,ZEN降解能力急剧下降。将米曲霉接种于受ZEN污染的豆粕中,28℃发酵120 h,经过发酵后豆粕中ZEN含量由1172.5 ng/g下降至66.0 ng/g,粗蛋白含量由45.1%提高至48.9%,大豆球蛋白和β-伴大豆球蛋白分别下降84.0%和92.5%。以上研究结果表明,米曲霉HAU491可以用于脱除豆粕中的ZEN,同时消减豆粕中的抗原蛋白和提高豆粕的饲用价值。
Zearalenone(ZEN)was a mycotoxin with estrogen-like effect that was commonly found in feed and feed ingredients,posing a serious threat to the health of livestock.We first investigated the ZENdegrading characteristics of Aspergillus oryzae HAU491,and then used the strain to detoxify ZEN in soybean meal by fermentation.The result showed that Aspergillus oryzae HAU491 could degrade 98.1%of ZEN at initial concentration of 2.5μg/mL in the reaction mixture within 3 h.The optimum pH range for the degradation of ZEN was 5.0-7.0.The 2 h degradation rate of ZEN by Aspergillus oryzae HAU491 maintained at more than 90%in the temperature range of 22-52℃.The active components responsible for ZEN degradation were located in the mycelium of Aspergillus oryzae HAU491,and the ZEN-degrading ability of the mycelium was almost completely abolished after four different treatments(100℃for 10 min,EDTA,proteinase K and SDS).Aspergillus oryzae was then inoculated into ZEN-contaminated soybean meal and fermented at 28℃for 120 h.After fermentation,the ZEN content of soybean meal was decreased from 1172.5 ng/g to 66.0 ng/g;the crude protein content was increased from 45.1%to 48.9%;and the soybean globulin andβ-companion soybean globulin contents were decreased by 84.0%and 92.5%,respectively.The above results indicated that Aspergillus oryzae HAU491 could be used to eliminate ZEN from soybean meal,and meanwhile decrease the antigenic protein contents and improve the feed value of soybean meal.
作者
陈晓爽
秦卓怡
许丹娜
张杏柯
张薇
郭永鹏
王志祥
CHEN Xiaoshuang;QIN Zhuoyi;XU Danna;ZHANG Xingke;ZHANG Wei;GUO Yongpeng;WANG Zhixiang(College of Animal Science and Technology,Henan Agricultural University,Henan Zhengzhou 450046,China;Animal Husbandry and Veterinary Quarantine and Prevention Center,Yibin District Management Committee,Henan Luoyang 471935,China)
出处
《饲料工业》
北大核心
2025年第2期142-147,共6页
Feed Industry
基金
中国博士后科学基金面上项目[2023M730998]
河南省科技攻关项目[222102110014]。
关键词
米曲霉
玉米赤霉烯酮
生物降解
发酵豆粕
抗原蛋白
Aspergillus oryzae
zearalenone
biodegradation
fermented soybean meal
antigenic protein
作者简介
陈晓爽,硕士,研究方向为动物营养与饲料科学;通信作者:王志祥,教授,博士生导师。
