摘要
为研究黄曲霉毒素B_(1)(AFB_(1))对肉鸡生长性能和肠道屏障的影响,将72只30日龄健康AA肉鸡随机分为2个组,每组4个重复,每个重复9只鸡。对照组饲粮AFB_(1)浓度为4.4μg/kg,AFB_(1)组饲粮AFB_(1)浓度为19.8μg/kg。试验期为19 d。结果显示:与对照组相比,AFB_(1)组料重比有升高趋势(P>0.05),空肠隐窝深度显著降低(P<0.05),十二指肠JAM-3 mRNA的表达有降低趋势(P>0.05),十二指肠MUC-2和回肠ZO-2基因表达显著降低(P<0.05),十二指肠IL-10、空肠IL-2和sIgA以及回肠sIgA含量显著提高(P<0.05),回肠IL-2和IL-10含量有升高趋势。综上所述,饲喂高AFB_(1)饲粮降低了肉鸡饲料转化率,破坏了肠道机械屏障功能,增加肠道炎症因子IL-2分泌,引起机体免疫反应。
The purpose of this study was to investigate the effects of aflatoxin B_(1)(AFB_(1))on production performance and gut barrier in broilers.A total of 7230-day-age AA broilers were randomly divided into two treatments with 4 replicates per treatment and 9 broilers per replicate.The control treatment were fed with the diet with 4.4μg/kg AFB_(1)and AFB_(1)treatment group were fed with the diet with 19.8μg/kg AFB_(1).The experiment lasted for 19 days.The results showed as followed:Compared with the control treatment,AFB_(1)treatment increased the feed weight ratio of chickens(P>0.05),reduced the depth of jejunal recess(P<0.05),decreased the expression of JAM-3 mRNA in duodenum tended(P>0.05),while the gene expression of MUC-2 in duodenum and ZO-2 in ileum decreased significantly(P<0.05).The contents of IL-10 in duodenum,IL-2 and sIgA in jejunum and sIgA in ileum increased significantly(P<0.05),while the contents of IL-2 and IL-10 in ileum tended to increase(P>0.05).In conclusion,feeding high level AFB_(1)diet could reduce feed conversion efficiency,destroy intestinal mechanical barrier function,increase intestinal inflammatory factor IL-2 secretion and cause immune response in broilers.
作者
李玉娟
刘磊
刘迎生
史强
王晓鹃
LI Yujuan;LIU Lei;LIU Yingsheng;SHI Qiang;WANG Xiaojuan(Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention,College of Animal Science and Technology,Shandong Agricultural University,Tai′an,Shandong 271018;Shandong Haiding Agriculture and Animal Husbandry Co.,Ltd.,Ji′nan,Shandong 250100)
出处
《中国家禽》
北大核心
2022年第5期63-68,共6页
China Poultry
基金
国家重点研发计划(2018YFE0128200)
国家自然科学基金项目(31672441)
现代农业产业技术体系建设专项资金(CAARS-40-K09)
泰山产业领军人才项目(201511023)
山东省重点研发计划项目(2019JZZY020602)
作者简介
李玉娟(1998-),女,硕士研究生,研究方向为饲料资源开发利用,E-mail:hbhslyj@163.com;通讯作者:王晓鹃(1983-),女,博士,副教授,主要从事家禽生态营养研究,E-mail:wangxj@sdau.edu.cn
