摘要
本实验对蛋鸡产蛋期使用含尼卡巴嗪饲料后鸡蛋中尼卡巴嗪(以4,4′-二硝基均二苯脲为残留标志物)的残留消除规律进行了研究,探讨制定尼卡巴嗪在鸡蛋中的临时最大残留限量(TMRL)、蛋鸡饲料中尼卡巴嗪的临时安全限量及修订弃蛋期的必要性,为进一步规范和指导饲料企业生产、养殖场合理用药,防止鸡蛋中尼卡巴嗪药物残留提供技术支持。实验选取28周龄海兰灰健康成年的已开产蛋鸡216羽,随机分为6个组(空白对照组、实验组Ⅰ、实验组Ⅱ、实验组Ⅲ、实验组Ⅳ、实验组Ⅴ),每组3个重复,每个重复12羽。6个组分别饲喂尼卡巴嗪添加剂量为0、100、200、300、400μg/kg和600μg/kg的饲料,连续饲喂30 d,停药后,全部饲喂基础日粮。鸡蛋采集时间为给药前1 d至停药后30 d,样品经前处理后用液相色谱-串联质谱法(LC-MS/MS)进行尼卡巴嗪残留标志物残留检测,分析尼卡巴嗪在鸡蛋中的残留消除规律。结果表明:给药期间,各实验组鸡蛋中尼卡巴嗪的残留量先迅速上升,分别于第10、8、8、10、7天达到最高残留量,之后有小幅度下降并逐渐趋于平缓。停药后,鸡蛋中尼卡巴嗪药物残留量快速下降,第11天后实验组(Ⅰ、Ⅱ组)的尼卡巴嗪残留浓度低于检测限(0.5μg/kg),第20天后实验组(Ⅲ、Ⅳ、Ⅴ组)的尼卡巴嗪残留浓度低于检测限。综上所述,尼卡巴嗪易在鸡蛋中残留且消除缓慢,根据尼卡巴嗪在鸡蛋中的残留消除规律,建议制定尼卡巴嗪在鸡蛋中的临时最大残留限量为100μg/kg,蛋鸡饲料中尼卡巴嗪的临时安全限量为600μg/kg。在蛋鸡饲料受到尼卡巴嗪污染时,鸡蛋中尼卡巴嗪的弃蛋期为33 d。
The experiment was aimed to study the residual elimination law of nicarbazine(regard 4,4′-dinitrocarbanilide as the residual marker of nicarbazine)in eggs of laying hens after using nicarbazine containing feed during the laying period,and to explore the necessity of establishing temporary maximum residual limits in eggs and temporary safety limits of nicarbazine in layer feed as well as revising the discontinuation period;Further standardize and guide the production of feed enterprises and the rational use of drugs in breeding farms to prevent the residue of nicarbazine in eggs.216 pieces of 28 weeks old Hailan Grey healthy adult laying hens were selected for the experiment and randomly divided into 6 groups(blank control group,experimental group I,experimental group II,experimental group III,experimental group IV,experimental group V),with three replicates in each group and twelve pieces in each replicate.The six groups were fed with the additional doses of nicarbazine at 0,100,200,300,400μg/kg and 600μg/kg for 30 consecutive days.After discontinuing the medication,all were fed with a basic diet.The egg collection time was from 1 day before administration to 30 days after discontinuation.After pre-treatment,the sample was subjected to liquid chromatography tandem mass spectrometry(LC-MS/MS)for residual marker identification of nicarbazine residues,and the residual elimination rules in eggs were analyzed.The results showed that during the administration period,the residual levels of nicarbazine in eggs of each experimental group first rapidly increased,reaching the highest residual amount on 10th,8th,8th,10th,and 7th days,respectively.After that,there was a slight decrease and gradually flattening out.After discontinuation of the drug,the residual concentration of nicarbazine in eggs rapidly decreased,and on the 11th day,the residual concentration of nicarbazine in experimental groups(I,II)was lower than the detection limit(0.5μg/kg).After 20 days,the residual concentration of nicarbazine in experimental groups(Ⅲ,Ⅳ,Ⅴ)was lower than the detection limit.In summary,nicarbazin is prone to residue and slow elimination in eggs.Based on the elimination pattern of nicarbazin residues in eggs,it was recommended to establish a temporary maximum residue limit of 100μg/kg for nicarbazin in eggs,a temporary safety limit of 600μg/kg in layer feed,When the layers'feed was contaminated with nicarbazine,the abandonment period of nicarbazine in eggs was 33 days.
作者
陈芳
李文静
卢丽枝
陈宝宇
曾云凤
刘昨
周炜炜
李伟胤
周振新
李俊
卜泽明
CHEN Fang;LI Wenjing;LU Lizhi;CHEN Baoyu;ZENG Yunfeng;LIU Zuo;ZHOU Weiwei;LI Weiyin;ZHOU Zhenxin;LI Jun;BU Zeming(Veterinary Drug Control Centre of Guangxi Zhuang Autonomous Region,Nanning,Guangxi Zhuang Autonomous Region 530001,China;Animal Husbandry Station of the Guangxi Zhuang Autonomous Region,Nanning,Guangxi Zhuang Autonomous Region 530022,China;Feed Research Institute Chinese Academy of Agricultural Sciences,Beijing 100000,China)
出处
《中国饲料》
北大核心
2024年第23期382-388,共7页
China Feed
基金
2022年广西农业科技自筹经费项目“饲料及禽蛋中尼卡巴嗪残留风险检测与安全评估”(Z202213)。
关键词
尼卡巴嗪
鸡蛋
药物残留
液相色谱-串联质谱法
nicarbazine
eggs
drug residues
liquid chromatography tandem mass spectrometry
作者简介
陈芳,研究生,畜牧师,研究方向为兽药、饲料及畜产品质量安全与检测技术,E-mail:75292307@qq.com;通讯作者:卜泽明,本科,畜牧师,研究方向为兽药、饲料及畜产品质量安全与检测技术,E-mail:369019041@qq.com;通讯作者:李俊,博士,研究员,研究方向为饲料加工与质量安全,E-mail:lijun08@caas.cn。
