摘要
目的:建立一种超高效液相色谱串联质谱同时测定鸡肉、鸡蛋中尼卡巴嗪、金刚烷胺、金刚乙胺残留量的检测方法。方法:样品用0.1%乙酸乙腈提取,加入无水硫酸钠和正己烷萃取,氮吹浓缩后经PSA净化,通过C18色谱柱分离,采用0.1%甲酸水-乙腈梯度洗脱,多反应监测模式,金刚烷胺、金刚乙胺采用电喷雾正离子(ESI+)扫描模式,尼卡巴嗪采用电喷雾负离子(ESI-)扫描模式,内标法定量。结果:3种物质在1~100 ng·mL^(-1)浓度下呈良好线性关系,相关系数均大于0.998,方法定量限均为0.5μg·kg^(-1)。样品分别在0.5μg·kg^(-1)、1.0μg·kg^(-1)、5.0μg·kg^(-1)加标量下,回收率为80.4%~112.3%。结论:本方法可简便快捷同时测定尼卡巴嗪、金刚烷胺、金刚乙胺3种物质,且灵敏度高、准确度好,可节省成本。
Objective: To establish an ultra performance liquid chromatography series mass spectrometry for the simultaneous determination of nicabazine, amantadine and rimantadine residues in chicken and eggs. Method: The sample was extracted from 0.1% acetonitrile acetate, anhydrous sodium sulfate and n-hexane extraction, nitrogen blowing and then purified by PSA, separated by C18column, 0.1% water-acetonitrile gradient elution, multi-reaction monitoring mode(MRM), amantadine and diamond ethylamine were normalized by electric spray. In the sub(ESI+) scanning mode,nicabayazine adopts electrospray anion(ESI-) scanning mode, which is quantitative by internal standard method. Result:Experiments show that the three substances have a good linear relationship at a concentration of 1~ 100 ng·mL^(-1), with correlation coefficients greater than 0.997, and the quantitative limit of the method is 0.5 μg·kg^(-1). The recovery rate of the sample is 80.4%~112.3% in the range of 0.5 μg·kg^(-1), 1.0 μg·kg^(-1)and 5.0 μg·kg^(-1)respectively. Conclusion: This method can easily and quickly determine the three substances of nicabazine, amantadine and amantadine at the same time. With high sensitivity and accuracy, it can save costs.
作者
欧阳思雨
蓝伟
贾佳
胡秋连
杜裕芳
OUYANG Siyu;LAN Wei;JIA Jia;HU Qiulian;DU Yufang(Testing Institute for Food Control of Jiangxi General Institute of Testing and Certification,Nanchang 330000,China)
出处
《现代食品》
2022年第23期138-141,147,共5页
Modern Food
关键词
尼卡巴嗪
金刚烷胺
金刚乙胺
高效液相-质谱
鸡肉
鸡蛋
nicarbazine
amantadine
high performance liquid phase-mass spectrometry
chicken
eggs
作者简介
欧阳思雨(1994-),女,本科,助理工程师,研究方向为食品安全检测;通信作者:蓝伟(1992-),男,本科,助理工程师,研究方向为食品安全检测。E-mail:577377494@qq.com。
