摘要
建立了同时测定尼卡巴嗪中4,4’-二硝基均苯二脲(DNC)和2-羟基-4,6-二甲基嘧啶(HDP)双组分的高效液相色谱法。实验采用安捷伦Poroshell 120 Bonus-RP色谱柱(4. 6mm×150mm,2.7μm),水和乙腈为流动相梯度洗脱,流速为0.8mL/min,柱温为40℃,HDP和DNC的检测波长均选择300 nm。试验结果表明HDP和DNC分别在23.3~34.9μg/m L和56.7~85.1μg/m L范围内线性关系良好,相关系数(r)分别为0.999 8和0.999 6。HDP平均回收率为100.4%,RSD为0.9%(n=9);DNC的平均回收率为99.2%,RSD为0.8%(n=9)。建立的方法简便、准确、可靠,可更好地控制尼卡巴嗪的质量。
A high performance liquid chromatographic method was established for the simultaneous determination of 4,4'-dinitrocarbanilide( DNC) and 2-hydroxy-4,6-dimethylpyrimidine( HDP) in Nicarbazin raw material drug.Analysis was performed on a Agilent Poroshell 120 Bonus-RP( 4.6 mm×150 mm,2.7 μm) column by using water-acetonitrile as mobile phase at a flow rate of 0. 8 m L/min and a column temperature of 40 ℃.The detecting wavelength of HDP and DNC were set at 300 nm. The results showed that a good linear relationship was accepted in the range of 23.3 ~ 34.9 μg/m L of HDP( r = 0.999 8) and 56.7 ~ 85.1μg/m L of DNC( r= 0.999 6).The average recovery of HDP was 100. 4%( n = 9) with RSD of 0. 9%.The average recovery of DNC was 99. 2%( n = 9) with RSD of 0. 8%. This method was convenient,accurate,and reliable. It was suitable to control the quality of Nicarbazin raw material drug.
作者
徐金雷
赵梅仙
马佳颖
肖进
梁景乐
齐鹏
吴家鑫
XU Jin-lei;ZHAO Mei-xian;MA Jia-ying;XIAO Jin;LIANG Jing-le;QI Peng;WU Jia-xin(China Animal Husbandry Industry Co.,Ltd. ,Beijing 100095,China)
出处
《化学试剂》
CAS
北大核心
2019年第7期716-720,共5页
Chemical Reagents
作者简介
徐金雷(1976-),男,江苏宿迁人,硕士,工程师,主要从事药品质量控制和研发工作,E-mail:2O04chem@163.com。
