摘要
肺癌是最常见肺部恶性肿瘤,居癌症相关死亡占比的首位。非血小板核糖核酸粒子(NPRCP)是一种新型再生粒子。通过观察不同浓度NPRCP对肺癌A549细胞的抑制率、细胞表面标志物及细胞周期的影响,旨在明确NPRCP对肺癌细胞的影响。指标包括NPRCP与A549细胞共培养后的细胞抑制率、流式细胞仪检测A549细胞周期和表面标志物CD133、CD44、CD24及ABCG2的表达率。结果显示,NPRCP对肺癌A549细胞存在抑制性的量-效和时-效关系,随着NPRCP浓度的递增,A549细胞上的CD44表达率显著降低。说明NPRCP可抑制肺癌A549细胞的增殖和CD44表达,NPRCP可能通过下调肺癌A549细胞的CD44表达而发挥抑制肿瘤增殖、转移的作用。
Lung cancer is the most common lung malignancy and the leading cause of cancer-related death.Non platelet RNA-containing particles(NPRCP)are a new type of regenerative particles.The aim of the study is to clarify the effects of NPRCP on lung cancer cells by observing the influence of NPRCP at different concentrations on inhibition ratio,cell surface markers,and cell cycle of A549 cells.The indicators are the cell inhibition ration after co-culture of NPRCP and A549 cells,and the cell cycle of A549 and the expression rate of CD133,CD44,CD24 and ABCG2 detected by flow cytometry.The results show inhibitory dose-response and time-dependent effect of NPRCP on lung cancer A549 cells.As the concentration of NPRCP increased,the CD44 expression rate on A549 cells decreased significantly.It suggests that NPRCP can inhibit the proliferation and CD44 expression of lung cancer A549 cells,and NPRCP may inhibit tumor proliferation and metastasis by downregulating the CD44 expression of A549 cells.
作者
张雪琳
云升
石秀换
ZHANG Xuelin;YUN Sheng;SHI Xiuhuan(Department of Oncology,the Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010030,China;Stem Cell Laboratory,the Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010030,China)
出处
《科技导报》
CAS
CSCD
北大核心
2024年第22期23-29,共7页
Science & Technology Review
基金
内蒙古医科大学科技百万联合项目(YKD2018KJBW(LH)032)
内蒙古医科大学研究生教育教学改革研究实践项目(YJG20191013215)
内蒙古医科大学附属医院重点实验室开放基金项目(2022NYFYSYS011)。
作者简介
张雪琳,主治医师,研究方向为肿瘤内科临床与分子机制,电子信箱:zx12769@163.com;通信作者:石秀换,主任医师,研究方向为肿瘤内科治疗与辅助治疗,电子信箱:shixiuhuan2023@163.com。
