摘要
目的探究miR-200b和miR-141在老年肺癌患者血清中表达水平及其对患者预后的影响。方法收集收治的64例老年肺癌患者为研究对象,另以50例健康体检老年人为健康对照组,通过qRT-PCR检测两组受试者血清中miR-200b和miR-141表达水平,分析其表达水平与老年肺癌患者临床病理特征和预后的关系。结果老年肺癌患者血清miR-200b表达水平显著低于健康对照组(P<0.05),miR-141显著高于健康对照组(P<0.05)。血清miR-200b和miR-141表达水平均与老年肺癌患者肿瘤分期、分化程度和淋巴结转移有关(P<0.05)。Kaplan-Meier分析结果显示miR-200b高表达组无进展生存率和总生存率显著高于低表达组(P<0.05);miR-141低表达组无进展生存率和总生存率显著高于高表达组(P<0.05)。COX分析结果显示miR-200b低表达和miR-141高表达及肿瘤分期为T3~T4是影响老年肺癌患者不良预后的独立危险因素。结论miR-200b在老年肺癌患者血清低表达,miR-141高表达,与老年肺癌患者预后有关,可作为老年肺癌患者预后判断的参考指标。
Objective To investigate the expressions of miR-200b and miR-141 in serum of elderly patients with lung cancer and their influences on prognosis.Methods Sixty-four elderly patients with lung cancer were enrolled,while another 50 healthy elderly patients were enrolled in the control group.The levels of miR-200b and miR-141 in the serum of the two groups were assessed by qRT-PCR.The correlations between their expression levels with the clinicopathological features and prognosis of elderly patients with lung cancer were analyzed.Results The expression level of serum miR-200b in elderly patients with lung cancer was significantly lower than that in control group(P<0.05),while miR-141 was significantly higher than that in control group(P<0.05).The expression levels of serum miR-200b and miR-141 were correlated with tumor stage,differentiation and lymph node metastasis(P<0.05).Kaplan-Meier analysis showed that the progression-free survival rate and overall survival rate in the miR-200b high expression group were significantly higher than those in the low expression group(P<0.05).The progression-free survival and overall survival rate in the miR-141 low expression group were significantly higher than those in the high expression group(P<0.05).COX analysis showed that low expression of miR-200b,high expression of miR-141,and tumor stage of T3-T4 were independent risk factors for poor prognosis in elderly patients with lung cancer.Conclusion MiR-200b is lowly expressed in elderly patients with lung cancer,while miR-141 is highly expressed,which is related to the prognosis of elderly patients with lung cancer,suggesting a reference index for the prognosis of elderly patients with lung cancer.
作者
王学林
刘逸翔
WANG Xue-lin;LIU Yi-xiang(Department of Respiratory and Critical Care Medicine,People's Hospital of Zhengzhou University,Zhengzhou 450003,Henan,China)
出处
《广东医学》
CAS
2019年第21期3040-3044,共5页
Guangdong Medical Journal
