摘要
本试验旨在探讨饲粮添加不同剂量艾蒿水提物(AAE)对杜寒杂交绵羊生长性能、营养物质表观消化率和肠道相关指标的影响。选取体重相近的健康3月龄杜寒杂交母羊32只,随机分为4组,每组8个重复,每个重复1只羊。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮的基础上添加500、1000和1500 mg/kg AAE的饲粮。预试期15 d,正试期60 d,分为试验前期(第1~30天)和试验后期(第31~60天)2个阶段。结果表明:1)随着AAE添加量的增加,试验前期,料重比(F/G)呈线性降低(P<0.05),干物质表观消化率呈二次变化(P<0.05),粗脂肪(EE)和中性洗涤纤维(NDF)表观消化率呈线性提高和二次变化(P<0.01);试验后期,EE、NDF和酸性洗涤纤维表观消化率呈线性提高和二次变化(P<0.05)。2)随着AAE添加量的增加,十二指肠α-淀粉酶和脂肪酶活性呈线性提高和二次变化(P<0.05),空肠脂肪酶活性呈线性提高和二次变化(P<0.01),回肠胰蛋白酶活性呈线性提高(P<0.05)。3)随着AAE添加量的增加,空肠隐窝深度(CD)呈线性降低(P<0.01)和二次变化(P<0.05);回肠绒毛高度(VH)呈线性提高(P<0.05),回肠CD呈二次变化(P<0.05),回肠VH/CD值呈线性提高和二次变化(P<0.05)。4)随着AAE添加量的增加,试验第60天,粪便总厌氧菌数量呈线性降低和二次变化(P<0.05),粪便双歧杆菌数量呈线性提高和二次变化(P<0.05)。由此可见,饲粮添加AAE可提高杜寒杂交绵羊营养物质表观消化率和肠道消化酶活性,改善肠道形态和微生物稳态,从而提高肠道功能,降低F/G;根据二次曲线回归分析,杜寒杂交绵羊饲粮中AAE添加量为1030 mg/kg时效果较好。
This experiment was conducted to investigate the effects of different supplemental levels of Artemisia argyi aqueous extract(AAE)on growth performance,nutrient apparent digestibility and intestinal related indices of Dorper×thin-tailed Han crossbred sheep.Thirty-two 3-month-old healthy Dorper×thin-tailed Han crossbred ewes with similar body weight were randomly divided into 4 groups with 8 replicates per group and 1 sheep per replicate.Sheep in the control group were fed a basal diet,and those in the experimental groups were fed the basal diet supplemented with 500,1000 and 1500 mg/kg AAE,respectively.The pre-trial period lasted for 15 days and the experimental period lasted for 60 days,which were divided into two stages as the early stage(days 1 to 30)and the late stage(days 31 to 60).The results showed as follows:1)with the increase of AAE supplemental level,in the early stage of the experiment,the ratio of feed to gain(F/G)was linearly decreased(P<0.05),the dry matter apparent digestibility was quadratically changed(P<0.05),and the apparent digestibilities of ether extract(EE)and neutral detergent fiber(NDF)were linearly increased and quadratically changed(P<0.01);in the late stage of the experiment,the apparent digestibilities of EE,NDF and acid detergent fiber were linearly increased and quadratically changed(P<0.05).2)With the increase of AAE supplemental level,the activities ofα-amylase and lipase in duodenum were linearly increased and quadratically changed(P<0.05),the lipase activity in jejunum was linearly increased and quadratically changed(P<0.01),and the trypsin activity in ileum was linearly increased(P<0.05).3)With the increase of AAE supplemental level,the crypt depth(CD)in jejunum was linearly decreased(P<0.01)and quadratically changed(P<0.05);the villus height(VH)in ileum was linearly increased(P<0.05),the CD in ileum was quadratically changed(P<0.05),and the VH/CD ratio in ileum was linearly increased and quadratically changed(P<0.05).4)With the increase of AAE supplemental level,on day 60 of the experiment,the total anaerobe number in feces was linearly decreased and quadratically changed(P<0.05),and the Bifidobacterium number in feces was linearly increased and quadratically changed(P<0.05).In conclusion,dietary supplementation of AAE can increase the nutrient apparent digestibility and intestinal digestive enzyme activity,improve intestinal morphology and microbial homeostasis,thereby improving intestinal function and decreasing F/G of Dorper×thin-tailed Han crossbred sheep.According to the quadratic regression analysis,the effect of dietary 1030 mg/kg AAE for Dorper×thin-tailed Han crossbred is better.
作者
李瑞臻
红雷
赵胡日查
高睿恒
岗根
于萍
赵英
史彬林
LI Ruizhen;HONG Lei;ZHAO Huricha;GAO Ruiheng;GANG Gen;YU Ping;ZHAO Ying;SHI Binlin(College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;Animal Husbandry Workstation,Horqin Left Wing Middle Banner,Tongliao City,Inner Mongolia,Baokang 029300,China;Agricultural and Livestock Product Quality and Safety Center of Inner Mongolia Autonomous Region,Hohhot 010000,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2024年第12期7854-7865,共12页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
内蒙古自治区直属高校基本科研业务费(BR22-13-13)。
关键词
艾蒿水提物
杜寒杂交绵羊
生长性能
营养物质表观消化率
肠道功能
Artemisia argyi aqueous extract
Dorper×thin-tailed Han crossbred sheep
growth performance
nutrient apparent digestibility
intestinal function
作者简介
李瑞臻(2000-),男,河北衡水人,硕士研究生,从事动物环境与营养的研究。E-mail:18832880815@163.com;通信作者:史彬林,教授,博士生导师,E-mail:shibinlin@yeah.net。
