摘要
目的:传统的竹笋壳转化燃料乙醇前期工艺采用的是化学水解法,该法存在严重的环境污染问题。为解决该问题,本文研究了利用白腐菌和霉菌同步发酵降解竹笋壳木质素及生物质转化产糖。方法:以冬笋壳为原料,以黄孢原毛平革菌(Phanerochaete chrysosporium)与里氏木霉(Trichoderma reesei)为发酵菌种,以木质素的降解率和可发酵糖的生成量为指标,通过响应曲面法分析各发酵参数对发酵效果的影响并最终确定最佳发酵工艺。结果:同步发酵的最佳工艺为接种量22%、温度24℃、发酵时间12 d,此时竹笋壳的木质素脱除率在57%,发酵糖生成量为264 mg/g。结论:本研究为包括竹笋壳在内的诸多农业废弃生物质原料的生物转化提供了一条可供借鉴的思路。
Objective:The traditional pre-process of converting bamboo shoot shells into fuel ethanol uses chemical hydrolysis method,which has serious environmental pollution problems.In order to solve this problem,the simultaneous fermentation of white rot and mold by white rot and mold is studied to degrade the lignin of bamboo shoot shells and biomass conversion to produce sugar.Methods:Using winter bamboo shoot shell as raw material,Phanerochaete chrysosporium and Trichoderma reesei as fermentation strains,the simultaneous fermentation of the two bacteria is carried out with the degradation rate of lignin and the production of fermentable sugar as indexes.The effect of fermentation parameters on fermentation effect is analyzed by response surface method,and the best fermentation process is determined finally.Results:The best process of simultaneous fermentation is 22%inoculum,24℃and 12 d fermentation time.At this time,the lignin removal rate of bamboo shoot shell is 57%,and the fermentation sugar production is 264 mg/g.Conclusion:This study provides a reference for the biotransformation of many agricultural waste biomass materials,including bamboo shoot shells.
作者
毕凡星
张建华
BI Fanxing;ZHANG Jianhua(Guangdong Bawei Biotechnology Corporation,Guangzhou 510450,China;College of Chemistry,Nanchang University,Nanchang 330003,China;Economic Crops Research Institute of Jiangxi Province,Nanchang 330202,China)
出处
《生物化工》
CAS
2024年第5期97-100,共4页
Biological Chemical Engineering
关键词
竹笋壳
黄孢原毛平革菌
里氏木霉
同步发酵
bamboo shoot shell
Phanerochaete chrysosporium
Trichoderma reesei
synchronous fermentation
作者简介
毕凡星(1985-),男,广东广州人,本科,工程师,研究方向为日用化工。
