摘要
旨在明确五味子醇甲对鸡大肠杆菌病的预防作用,将180只1日龄蛋鸡随机分为6组:空白对照组、E.coli感染组、50 mg·kg^(-1)五味子醇甲组、100 mg·kg^(-1)五味子醇甲组、200 mg·kg^(-1)五味子醇甲组和抗生素组(氟苯尼考)。饲养至14 d时,按分组剂量开始给药,给药5 d后,除空白对照组外其余各组均腹腔接种大肠杆菌菌液,检测雏鸡体重、免疫器官指数、脾病理变化和超微结构、血细胞数、血清炎症因子、抗体水平和免疫球蛋白的变化。结果表明,五味子醇甲能够提高患病雏鸡体重,显著降低脾指数,减轻由于大肠杆菌感染导致的脾组织结构以及细胞中细胞核和线粒体的损伤,显著降低患病鸡的白细胞数量以及血清中TNF-α、IL-1β、IL-6和IL-8水平,并显著升高IgM、IgG和NDV的抗体水平。综上所述,五味子醇甲能够通过调节免疫对鸡大肠杆菌病起抗病作用。
To investigate the preventive effect of schisandrin A against chicken colibacillosis,180 one-day-old chicks were divided into the following groups:control group,Escherichia coli infection group,50 mg·kg^(-1) schisandrin A group,100 mg·kg^(-1) schisandrin A group,200 mg·kg^(-1) schisandrin A group,and antibiotic group(florfenicol).After 5 days of administration,except for the control group,all other groups were intraperitoneally inoculated with E.coli suspension.The chicks'body weight,immune organ index,spleen histopathological and ultrastructural changes,blood cell count,serum inflammatory factors,antibody levels,and immunoglobulin changes were evaluated.The results demonstrated that pre-feeding schisandrin A improved the body weight of infected chicks,significantly reduced the spleen index,alleviated spleen tissue damage and cellular nuclear and mitochondrial injuries caused by E.coli infection.Furthermore,200 mg·kg^(-1) schisandrin A significantly reduced the white blood cell count in infected chickens,while both 100 mg·kg^(-1) and 200 mg·kg^(-1) schisandrin A groups significantly decreased the levels of TNF-α,IL-1β,IL-6,and IL-8 in the serum of infected chickens,and significantly increased the antibody levels of IgM,IgG,and NDV.In summary,schisandrin A exerted a disease-resistant effect against avian colibacillosis by modulating the immune response.
作者
包佳鹭
张妍
王晓丹
BAO Jialu;ZHANG Yan;WANG Xiaodan(College of Chinese Veterinary Medicine,Hebei Agricultural University,Baoding 071001,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2024年第10期4735-4746,共12页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
河北省重点研发计划(21326602D)。
关键词
五味子醇甲
鸡大肠杆菌病
免疫
schisandrin A
chicken colibacillosis
immunity
作者简介
包佳鹭(1994-),女,河北廊坊人,博士生,主要从事中兽医学研究,E-mail:15383066910@163.com;通信作者:王晓丹,主要从事中兽医学研究,E-mail:wangxiaodan_wxd@126.com。
