摘要
目的研究人参皂苷Rg1和Rb1改善慢性不可预测应激诱导大鼠的抑郁样和焦虑样行为的作用及比较。方法SPF级SD雄性大鼠70只,适应5 d后进行糖水偏爱实验检测,根据糖水偏爱指数将动物分为7组,即对照组、模型组、氟西汀组、人参皂苷Rg124 mg/kg剂量组、人参皂苷Rg148 mg/kg剂量组、人参皂苷Rb133 mg/kg剂量组、人参皂苷Rb167 mg/kg剂量组。除对照组外,其余大鼠每天随机接受1~2种不同的刺激,造模时间共35 d。于第36天进行糖水偏爱、旷场实验、新奇环境摄食抑制实验、大鼠高架十字迷宫实验、强迫游泳等行为学实验,考察其抗抑郁、抗焦虑作用。采用ELISA法测定大鼠血清和海马IL⁃1β、IL⁃6、TNF⁃α炎症因子水平,血清皮质酮(CORT)水平。结果与模型组相比,人参皂苷Rg1、Rb1组大鼠糖水偏爱指数提升,强迫游泳不动时间显著减少,人参皂苷Rg148 mg/kg剂量组新奇抑制摄食潜伏期显著减少,人参皂苷Rg1(24和48 mg/kg)剂量组开臂时间的比例显著上升,人参皂苷Rg1、Rb1两个剂量组血清中皮质酮的含量显著减少,人参皂苷Rg124 mg/kg剂量组血清中IL⁃1β和IL⁃6的水平显著降低,人参皂苷Rb133 mg/kg剂量组血清中TNF⁃α和IL⁃6的水平显著降低,人参皂苷Rg1(48 mg/kg)、Rb1(67 mg/kg)剂量组海马中IL⁃1β、IL⁃6和TNF⁃α的含量显著降低。结论两种人参皂苷均可能通过调节HPA轴、抑制神经炎症,从而改善慢性不可预测应激致大鼠抑郁、焦虑样行为,此外人参皂苷Rg1的抗焦虑作用优于Rb1。
Objective To compare the effects of ginsenosides Rg1 and Rb1 on depression⁃and anxiety⁃like behaviors in chronic unpredictable stress⁃induced rats.Methods Seventy male SPF grade SD rats were tested for sugar and water preference after 5 days of adaptation and divided into seven groups according to their preference index:a control group,model group,fluoxetine hydrochloride group,ginsenoside Rg124 mg/kg group,ginsenoside Rg148 mg/kg group,ginsenoside Rb133 mg/kg group,and ginsenoside Rb167 mg/kg group.All rats,except for the control group,were subjected randomly to one or two different stimulating factors every day for a total of 35 days.On the 36th day,behavioral experiments including sugar and water preference,open field,novel environment feeding inhibition,elevated cross maze,and forced swimming experiments were conducted to investigate the anti⁃depression and anti⁃anxiety effects of the treatments.Serum and hippocampal levels of interleukin(IL)⁃1β,IL⁃6,tumor necrosis factor(TNF)⁃αand serum corticosterone were measured by enzyme⁃linked immunosorbent assay.Results Compared with the model group,ginsenoside Rg1 and Rb1 significantly increased sucrose consumption in the sucrose preference test and decreased immobility in the forced swimming test.Ginsenoside Rg1(48 mg/kg)significantly reduced the latency to eat in the novelty⁃suppressed feeding test,and ginsenoside Rg1(24 and 48 mg/kg)significantly increased the percentage of open arm entries and time in the elevated cross maze test.Serum corticosterone levels were significantly decreased in the ginsenoside Rg1 and Rb1 groups,serum IL⁃1βand IL⁃6 levels were significantly decreased in the ginsenoside Rg1(48 mg/kg)group,serum TNF⁃αand IL⁃6 levels were significantly decreased in the ginsenoside Rb1(33 mg/kg)group,and IL⁃1β,IL⁃6,and TNF⁃αlevels in the hippocampus were significantly decreased in the ginsenoside Rg1(48 mg/kg)and Rb1(67 mg/kg)groups.Conclusions Both ginsenosides can regulate the hypothalamic⁃pituitary⁃adrenal axis and inhibit neuroinflammation,improving depression⁃and anxiety⁃like behaviors in rats induced by chronic unpredictable stress.Ginsenoside Rg1 has a significantly better anti⁃anxiety effect than Rb1.
作者
贝雪怡
姜宁
姚彩虹
张亦文
孙欣然
罗燕琴
李亮
谢梦洲
刘新民
BEI Xueyi;JIANG Ning;YAO Caihong;ZHANG Yiwen;SUN Xinran;LUO Yanqin;LI Liang;XIE Mengzhou;LIU Xinmin(Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Engineering and Technology Research Center of Medicinal and Edible Homologous Functional Food,Hunan University of Chinese Medicine,Changsha 410208;Key Laboratory of TCM Cardiopulmonary Syndrome Differentiation and Medicinal Diet Diet Therapy,Hunan University of Chinese Medicine,Changsha 410208;Institute of Medicinal Plants,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193;Institute of New Drug Technology,Ningbo University,Ningbo 315000)
出处
《中国比较医学杂志》
CAS
北大核心
2024年第7期68-78,共11页
Chinese Journal of Comparative Medicine
基金
中国医学科学院创新工程(2021⁃1⁃I2M⁃034)。
作者简介
贝雪怡(1997-),女,硕士研究生,研究方向:中医药膳学。E⁃mail:294773448@qq.com;共同通信作者:刘新民(1962-),男,教授,博士生导师,研究方向:中药神经药理及实验方法。E⁃mail:liuxinmin@hotmail.com;共同通信作者:李亮(1980-),男,博士,教授,博士生导师,研究方向:中医辨证学与数字中医药。E⁃mail:superliliang@126.com。
