摘要
目的构建心肌梗死大鼠模型,观察曲美他嗪对模型大鼠心肌细胞凋亡、自噬以及心功能的保护作用。方法60只SPF级成年雄性SD大鼠使用随机数字表法分为假手术组、对照组和曲美他嗪组。应用TUNEL法检测心肌细胞凋亡,并计算凋亡指数(PI),Western Blot检测心肌细胞自噬蛋白。彩超测定左心室收缩末期内径(LVESD)、短轴缩短分数(FS)和射血分数(EF)。结果假手术组、对照组、曲美他嗪组心肌细胞PI值分别为(0.76±0.23)%、(21.52±6.57)%和(12.38±2.15)%,心肌自噬蛋白LC3Ⅱ相对灰度值分别为0.17±0.03、0.46±0.12、0.68±0.15,3组间PI值和LC3Ⅱ相对灰度值比较差异有统计学意义(P<0.01)。术后第28天,假手术组LVESD、FS和EF与术前比较差异无统计学意义(P>0.05),对照组和曲美他嗪组LVESD较术前升高(P<0.05),FS和EF较术前减低(P<0.05)。曲美他嗪组LVESD术后第28天低于对照组(P<0.05),曲美他嗪组FS和EF术后第28天高于对照组(P<0.05)。结论曲美他嗪有助于心肌梗死大鼠心功能恢复,减低心肌细胞凋亡和增加心肌自噬。
Objective To observe the effects of trimetazidine on cardiac function,cardiomyocyte apoptosis and autophagy in rats with myocardial infarction.Methods Rat model of myocardial infarction was established.Sixty SPF-grade adult male Sprague-Dawley(SD)rats were randomly divided into sham-operated group,control group and trimetazidine group.TUNEL was used to detect cardiomyocyte apoptosis.Myocardial autophagy protein was detected by Western blot.Left ventricular end-systolic diameter(LVESD),fractional shortening(FS),and ejection fraction(EF)were detected by color Doppler ultrasound.Results The PI values of myocardial cells in sham operation group,control group and trimetazidine group were(0.76±0.23)%,(21.52±6.57)%,and(12.38±2.15)%,respectively.The relative gray values of myocardial autophagic protein LC3Ⅱwere(0.17±0.03),(0.46±0.12),and(0.68±0.15)in sham operation group,control group and trimetazidine group.There were significant differences in PI value and relative gray value of LC3Ⅱamong the three groups(P<0.01).At 28 days after the operation,LVEDD was significantly increased in the control group and trimetazidine group(P<0.05),while FS and EF were reduced significantly(P<0.05).The LVEDD value of the trimetazidine group was significantly lower than that of the control group at 28 days after surgery(P<0.05),and the FS and EF values of the trimetazidine group were significantly higher than those of the control group at 28 days after surgery(P<0.05).Conclusion Trimetazidine is helpful for the recovery of cardiac function in myocardial infarction rats,reduces myocardial cell apoptosis and increases myocardial autophagy,which may be its mechanism of action.
作者
黎云
高日扬
林小亮
杨静
LI Yun;GAO Riyang;LIN Xiaoliang;YANG Jing(Fourth People′s Hospital of Haikou,Haikou 571100,Hainan,China)
出处
《中西医结合心脑血管病杂志》
2020年第3期434-436,共3页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
关键词
心肌梗死
曲美他嗪
心肌凋亡
心肌自噬
心功能
myocardial infarction
trimetazidine
myocardial apoptosis
myocardial autophagy
cardiac function
作者简介
黎云,E-mail:13307682123@163.com。
