摘要
为研究不同产地C2F等级烤烟表面细菌微生物的组成和结构,利用高通量测序技术测试了6个不同产地的C2F等级烤烟在醇化初期烟叶表面可培养细菌的组成和结构。结果表明:6个不同产地的烟叶样品表面分别检测到55、39、33、26、24和15个OTU,其中6个烟叶样品共同的OTU为6个。在属分类水平上,大部分烟叶表面可培养的优势细菌微生物是芽孢杆菌属(Bacillus),部分烟叶表面活跃着肠杆菌属(Enterobacter),伯克霍尔德菌属(Burkholderia)和罗尔斯通菌属(Ralstonia)。不同品种和产地的烤烟表面细菌结构具有明显差异,同一品种不同产地的烤烟表面细菌结构也存在明显差异。研究结果为利用细菌微生物醇化不同产区烟叶,提高烟叶品质、优化烟叶醇化流程提供理论依据。
In order to study the composition and structure of bacteria on the surface of C2F flue-cured tobacco from different regions,the composition and structure of culturable bacteria on the surface of tobacco leaves from 6 different regions at the early stage of aging were tested using high throughput sequencing technology.The results showed that 55,39,33,26,24 and 15 OTUs were detected on the surface of tobacco samples from six different origins,respectively,and 6 OTUs were shared by tobacco samples from six different origins.At the genus classification level,the dominant bacterial microorganism that could be cultured on the surface of most tobacco leaves was Bacillus,and Enterobacter,Burkholderia and Ralstonia were active in some flue-cured tobacco surfaces.There were obvious differences in the types of bacteria on the surface of flue-cured tobacco leaves from different varieties and producing areas,and also from different producing areas of the same variety.The research results provide a theoretical basis for using bacteria to age tobacco leaves from different producing areas,improve the quality of tobacco leaves,and optimize the tobacco leaf aging process.
作者
汪季涛
王浩军
舒俊生
沈忱
朱英华
葛高飞
WANG Jitao;WANG Haojun;SHU Junsheng;SHEN Chen;ZHU Yinghua;GE Gaofei(Technical Center,China Tobacco Anhui Industrial Co.,Ltd.,Hefei 230088;School of Agronomy,Anhui Agricultural University,Hefei 230036;Biotechnology Center,Anhui Agricultural University,Hefei 230036)
出处
《安徽农业大学学报》
CAS
CSCD
2024年第3期510-515,共6页
Journal of Anhui Agricultural University
基金
安徽中烟工业有限责任公司科技项目(2020121)资助。
关键词
烤烟
细菌群落结构
高通量测序
flue-cured tobacco
bacterial community structure
high-throughput sequencing
作者简介
汪季涛,博士,高级农艺师。E-mail:22877515@qq.com;通信作者:葛高飞,博士,研究员。E-mail:gegaofei@ahau.edu.cn。
