摘要
为探究细菌微生物群落在杨树湿心材发病过程中的作用,以江汉平原6年生华石2号黑杨湿心材和正常材为研究对象,采用高通量测序对16S rRNA基因V5―V7区进行测序,对测序列结果进行信息学分析,利用主成分分析、α多样性指数分析和微生物网络分析技术等方法,探究6年生黑杨湿心材和正常材细菌微生物群落结构和组成差异。结果表明:6年生华石2号黑杨湿心材和正常材细菌微生物群落属水平上,劳尔氏菌属和拟杆菌属的细菌丰度最高,拟杆菌属、多形单胞菌属和氢孢菌属在湿心材中占比显著高于正常材(P<0.05)。微生物群落功能分析显示,劳尔氏菌属多为植物病原菌,在湿心材中广泛存在。α多样性指数分析表明,湿心材群落多样性和物种分布均匀度高于正常材,物种总数低于正常材。共现网络研究表明,黑杨正常材细菌微生物之间存在较多的竞争和拮抗作用,群落较不稳定,湿心材细菌间存在更多的协同和互生关系,微生物群落较稳定。关键微生物分析表明,6年生黑杨湿心材中关键细菌微生物是Aquabacterium、WCHB1-32等,6年生黑杨正常材中关键细菌微生物是Pleomorphomonas、Dysgonomonas,这些关键微生物在稳定湿心材微生物网络结构中发挥着重要作用。以上结果表明,黑杨湿心材形成受多方面因素的影响,是由微生物群落共同作用产生的结果。
The wet heartwood and normal wood of 6-year-old Populus deltoides Bartr.‘Huashi2’in the Jianghan Plain were used to study the role of bacterial and microbial communities in the pathogenesis in the wet heartwood of Populus.High-throughput sequencing was used to sequence the V5-V7 region of the 16S rRNA gene.The results of sequencing were analyzed with bioinformatics.Methods including principal component analysis,α-diversity index analysis,and microbial network analysis were used to study the differences in the structure and composition of the bacterial and microbial communities in the wet heartwood and normal wood of 6-year-old Populus deltoides Bartr.The results of principal component analysis showed that the bacterial abundance of the genera Ralstonia and Bacteroides was the highest,while the proportion of the genera Bacteroides,Pleomorphomonas and Hydrogenispora in wet heartwood was significantly higher than that in normal wood(P<0.05)at the genus level of the bacterial and microbial communities in the wet heartwood and normal wood of 6-year-old Populus deltoides Bartr.The results of analyzing the functions of microbial communities showed that Ralstonia were mostly phytopathogenic bacteria,which widely existed in wet heartwood.The results of analyzing the alpha diversity index showed that the diversity and species distribution uniformity of the community in the wet heartwood were higher than those in the normal wood,and the total number of species in the wet heartwood was lower than that in the normal wood.The results of analyzing the co-occurrence network showed that there was a lot of competition and antagonism between bacteria and microorganisms in the normal wood of Populus deltoides Bartr,and the community was relatively unstable.There were more synergistic and mutualistic relationships among bacteria in wet heartwood,and the microbial community was relatively stable.The results of analyzing key microorganisms showed that the key bacterial microorganisms in the wet heartwood of 6-year-old Populus deltoides Bartr.were Aquabacterium,WCHB1-32,etc.,and the key bacterial microorganisms in the normal heartwood of 6-year-old Populus deltoides Bartr.were Pleomorphomonas,Dysgonomonas,etc.These key microorganisms played an important role in stabilizing the structure of microbial network in the wet heartwood.It is indicated that the formation of wet heartwood in Populus deltoides Bartr.is affected by multiple factors and is the result of the joint action of microbial communities.
作者
周靖钰
黄国伟
张亚东
祁高富
张新叶
ZHOU Jingyu;HUANG Guowei;ZHANG Yadong;QI Gaofu;ZHANG Xinye(College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Hubei Academy of Forestry Sciences,Wuhan 430079,China)
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2024年第4期221-229,共9页
Journal of Huazhong Agricultural University
基金
国家重点研发计划项目(2021YFD2201202)。
关键词
黑杨
湿心材
高通量测序
细菌
微生物群落
Populus dehoides Bartr
wet heartwood
high-throughput sequencing
bacteria
microbial community
作者简介
周靖钰,E-mail:zhjingyu06@163.com;通信作者:张新叶,E-mail:1641135733@qq.com。
