摘要
以‘韩研28-6’黄瓜种子为试材,清水为对照(CK),用7.2×10^(7)、7.2×106、7.2×10^(5)、3.6×10^(5)CFU·mL^(-1)浓度枯草芽孢杆菌BSCY-1菌悬液分别对黄瓜种子进行浸种处理和幼苗灌根处理,研究不同浓度BSCY-1菌悬液对黄瓜种子萌发、幼苗形态指标和生理特性的影响,初步明确草莓叶中分离出的内生枯草芽孢杆菌BSCY-1对黄瓜的促生效应,以期为枯草芽孢杆菌在肥料中添加提供参考依据。结果表明:与CK相比,灌根7、14 d后3.6×10^(5)CFU·mL^(-1)BSCY-1处理显著提高黄瓜幼苗叶面积、鲜质量;BSCY-1菌悬液灌根7、14 d,7.2×10^(5)、3.6×10^(5)CFU·mL^(-1)BSCY-1处理的过氧化氢酶、过氧化物酶活性显著高于CK;灌根14、21 d,7.2×10^(5)、3.6×10^(5)CFU·mL^(-1)BSCY-1处理的超氧化物歧化酶活性显著高于CK;灌根14 d,3.6×10^(5)CFU·mL^(-1)BSCY-1处理的可溶性淀粉含量、可溶性糖含量、叶绿素含量明显高于对照。综上所述,枯草芽孢杆菌BSCY-1可改善黄瓜幼苗的生理生化特征,进而促进幼苗生长,尤其是3.6×10^(5)CFU·mL^(-1)BSCY-1的促生效果最为明显。
The cucumber seeds of‘Hanyan 28-6’were used as test materials,water was used as control(CK),cucumber seeds were soaked and irrigated with 7.2×10^(7),7.2×106,7.2×10^(5),3.6×10^(5)CFU·mL^(-1)four concentrations of B.subitilis BSCY-1 bacterial suspension,to explore the effects of different concentrations of BSCY-1 bacterial suspension on seed germination,as well as morphological and physiological characteristics of seedlings.The growth-promoting effect of endophytic B.subtilis BSCY-1 isolated from strawberry leaves on cucumber was preliminarily clarified,in order to provide reference for the addition of B.subtilis in fertilizer.The results showed that compared with CK,after 7 days and 14 days of root irrigation,3.6×10^(5)CFU·mL^(-1)BSCY-1 treatment significantly increased the leaf area and fresh weight of cucumber seedlings.After 7 days and 14 days of root irrigation with BSCY-1,the activities of CAT and POD of 7.2×10^(5)CFU·mL^(-1)and 3.6×10^(5)CFU·mL^(-1)treatments were significantly higher than those in CK.SOD activity of 7.2×10^(5),3.6×10^(5)CFU·mL^(-1)BSCY-1 treatments were significantly higher than that of CK after 14 days and 21 days.After 14 days of irrigation with BSCY-1,the soluble starch content,soluble sugar content and chlorophyll content of 3.6×10^(5)CFU·mL^(-1)treatment were significantly higher than those of the control.In summary,B.subtilis BSCY-1 could improve the physiological and biochemical characteristics of cucumber seedlings,thereby promoting seedling growth,especially the growth-promoting effect of 3.6×10^(5)CFU·mL^(-1)BSCY-1 treatment was the most obvious.
作者
谷清义
王妍佳
李梦楠
李蒙
刘松虎
申君
GU Qingyi;WANG Yanjia;LI Mengnan;LI Meng;LIU Songhu;SHEN Jun(School of Pharmacy,Xinyang Agriculture and Forestry University,Xinyang,Henan 464000;Horticulture College,Xinyang Agriculture and Forestry University,Xinyang,Henan 464000)
出处
《北方园艺》
CAS
北大核心
2024年第10期17-23,共7页
Northern Horticulture
基金
河南省高等学校重点科研资助项目(21B210009)
信阳农林学院高水平科研孵化器建设资助项目(FCL202101)
信阳农林学院青年基金资助项目(QN2021060)。
关键词
枯草芽孢杆菌
黄瓜
促生效应
Bacillus subtilis
cucumber
growth promoting effect
作者简介
第一作者:谷清义(1982-),男,硕士,助教,现主要从事作物栽培与病虫害防治等研究工作。E-mail:271490029@qq.com;责任作者:申君(1985-),女,博士,副教授,现主要从事蔬菜栽培与病虫害防治等研究工作。E-mail:shenjun996@163.com.
