摘要
目的:探究椴树蜜对食源性致病菌的体外抑菌活性。方法:采用牛津杯抑菌试验和肉汤稀释法,将椴树蜜与灭菌蒸馏水充分混合后得到不同质量浓度的样品。用灭菌的蒸馏水作对照,测定其对金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌和乙型副伤寒沙门菌的抑菌能力和最小抑菌浓度。结果:不同质量浓度的样品对金黄色葡萄球菌、大肠埃希菌及乙型副伤寒沙门菌的抑菌作用非常明显,但均未对铜绿假单胞菌产生明显的抑菌圈。样品质量浓度为20%(w/V)时,对金黄色葡萄球菌的抑菌性最强,抑菌圈直径达(26.74±0.06)mm。椴树蜜对金黄色葡萄球菌、大肠埃希菌及乙型副伤寒沙门菌的最小抑菌浓度为10%、20%、20%。结论:椴树蜜对金黄色葡萄球菌、大肠埃希菌和乙型副伤寒沙门菌均有抑菌作用,且对金黄色葡萄球菌的抑菌能力最强。该研究结果可为椴树蜜作为抑菌剂的进一步开发和利用提供理论依据。
Objective:To explore the antibacterial activity of linden honey against food borne pathogens in vitro.Methods:According to the Oxford cup bacteriostatic test and broth dilution method,samples with different mass concentrations were obtained after linden honey was fully mixed with sterilized distilled water.The sterilized distilled water was used as a control to determine its bacteriostatic ability and minimum inhibitory concentration against Staphylococcus aureus(S.aureus),Pseudomonas aeruginosa(P.aeruginosa),Escherichia coli(E.coli) and Salmonella paratyphi B(S.paratyphi B).Results:The antibacterial effects of samples with different mass concentrations on S.aureus,E.coli,and S.paratyphi B were very obvious,but none of them showed a significant inhibitory zone against P.aeruginosa.When mass concentration of the sample is 20%(w/V),the antibacterial activity against S.aureus is the strongest,with a diameter of(26.74±0.06)mm.The minimum inhibitory concentrations of linden honey against S.aureus,E.coli,and S.paratyphi B were 10%,20% and 20%.Conlusion:Linden honey has antibacterial effects on S.aureus,E.coli,and S.paratyphi B,and has the highest antibacterial ability against S.aureus.This study provides a theoretical basis for the further development and utilization of linden honey as an antibacterial agent.
作者
武鑫
陈子龙
郑敏
罗叶丽
卫延明
郭莉君
WU Xin;CHEN Zilong;ZHENG Min;LUO Yeli;WEI Yanming;GUO Lijun(Weinan Inspection and Testing Research Institute,Weinan 714000,China)
出处
《食品科技》
CAS
北大核心
2024年第2期67-71,共5页
Food Science and Technology
基金
渭南市科技计划重点研发计划项目(2022ZDYFJH-168)。
关键词
椴树蜜
抑菌活性
食源性致病菌
linden honey
antibacterial activity
foodborne pathogenic bacteria
作者简介
武鑫(1989-),女,陕西潼关人,硕士,工程师,研究方向为食品和药品微生物检验检测。
