摘要
采集了新疆8个地区15个牛场421份4月龄内犊牛的肛拭子,应用RT-PCR方法扩增牛冠状病毒(bovine coronavirus,BCoV)N基因和S基因,对S基因蛋白序列进行遗传进化分析。结果显示,BCoV个体核酸阳性率为19.91%(67/421),7~30日龄犊牛阳性率为33.59%(44/131),有临床腹泻症状犊牛的核酸阳性率为18.21%(57/313),伊犁地区最高34.88%(15/43);获得3条S基因序列,全长分别为4092、4092、4093bp,与参考序列核苷酸同源性为98.88%~99.46%;遗传进化分析显示3条S基因与国内四川毒株SWUN/NMG-13(MW711304.1)和BCOVChina/SWUN/LN4(MK095182.1)核苷酸同源性最近,为99.3%~99.4%,均属于GⅡ型;与国内外17株参考毒株相比,所测毒株存在23个氨基酸位点突变,均在I113V、D115A、S501P和T510S位点出现突变。结果表明,新疆地区1~4月龄犊牛普遍存在BCoV感染,且S蛋白存在部分突变。本研究揭示了新疆牛场犊牛BCoV不同月龄和地域流行分布及其S蛋白的变异特征,为BCoV防制提供了依据。
To understand the epidemic distribution and genetic variation of bovine coronavirus(BCoV)in calves in intensive farms in Xinjiang,421anal swabs of calves within 4months of age from 15farms in 8regions of Xinjiang were collected.The N gene and S gene of BCoV were amplified by RT-PCR,and the genetic evolution of S gene protein sequence was analyzed.The results showed that the positive rate of BCoV individual nucleic acid was 19.91%(67/421),the positive rate of 7-30days of age was 33.59%(44/131),the positive rate of clinical diarrhea calf nucleic acid was 18.21%(57/313),and the highest in Ili area was 34.88%(15/43).Three S gene sequences were obtained,with a total length of 4092,4092and 4093bp,respectively.The nucleotide homology with the reference sequence was 98.88%-99.46%.Genetic evolution analysis showed that the three S genes had the closest nucleotide homology with the domestic Sichuan strain SWUN/NMG-13(MW711304.1)and BCoV-China/SWUN/LN4(MK095182.1),ranging from 99.3%to 99.4%,all belonging to GⅡtype.Compared with 17reference strains at home and abroad,there were 23amino acid site mutations in the tested strains,all of which occurred at I113V,D115A,S501Pand T510Ssites.The results showed that BCoV infection was common in calves aged 1―4months in Xinjiang,and there were some mutations in S protein.The study revealed the epidemic distribution of BCoV in different months and regions of calves in Xinjiang cattle farms and the variation characteristics of S protein,which provides a basis for BCoV prevention and control.
作者
贾开文
操义恒
胡亚辉
党士荣
钟发钢
赵文娟
吴桐忠
韩猛立
张星星
张倩
周霞
黄新
JIA Kaiwen;CAO Yiheng;HU Yahui;DANG Shirong;ZHONG Fagang;ZHAO Wenjuan;WU Tongzhong;HANG Mengli;ZHANG Xingxing;ZHANG Qian;ZHOU Xia;HUANG Xin(State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding,Xinjiang Academy of Agricultural Reclamation Sciences,Shihezi,Xinjiang832000,China;College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang832003,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2024年第4期664-669,共6页
Chinese Journal of Veterinary Science
基金
新疆生产建设兵团重点领域科技攻关计划资助项目(2019AB029)
省部共建绵羊遗传改良与健康养殖国家重点实验室建设-创新环境建设和能力提升计划资助项目(2020CA001)
中央级公益性科研院所基本科研业务费院级统筹资助项目(Y2022LM21)
新疆生产建设兵团重点领域科技攻关计划资助项目(2021AB012)
兵团第三师市财政科技计划资助项目(KJ2022CX01)。
关键词
牛冠状病毒
流行病学调查
S基因
遗传进化分析
bovine coronavirus
epidemiological survey
S gene
genetic evolution analysis
作者简介
贾开文(1997-),男,硕士研究生。;通信作者:黄新,E-mail:ahx512@163.com;通信作者:周霞,E-mail:zhouxia0993@aliyun.com。
