摘要
以天花粉粗多糖(TRP)和壳聚糖(CS)为基质、柠檬酸(CA)为交联剂制备复合水凝胶作为伤口敷料促进小鼠皮肤伤口快速愈合。采用水提醇沉法提取天花粉粗多糖、三氯乙酸法除蛋白进行纯化;通过一步冻融法制备TRP水凝胶;通过傅里叶变换红外光谱(FT-IR)、扫描电镜(SEM)和质构分析揭示了TRP水凝胶的结构、形貌和机械性能;测定了TRP抗氧化能力以及TRP水凝胶的溶胀性能、溶血率;采用小鼠全皮层损伤模型研究了TRP水凝胶促进伤口愈合的能力。结果表明TRP水凝胶最大抗压强度为13.79 kPa,溶胀度为12.41;TRP和TRP水凝胶均具有较低的溶血率,TRP溶血率最高为1.29%,TRP6水凝胶(TRP含量为6 mg/mL)的溶血率为2.36%;TRP表现出良好的抗氧化活性,能有效清除多种自由基;在小鼠全皮层损伤修复实验中,TRP水凝胶组小鼠的愈合速度最快,H&E染色结果表明TRP水凝胶能更好的促进小鼠皮肤伤口处的毛囊再生和皮肤组织重建。研究表明TRP水凝胶在伤口敷料应用方面具有巨大潜力。
The composite hydrogel was prepared using trichosanthin polysaccharide(TRP),chitosan(CS),and citric acid(CA)as a crosslinking agent and utilized as a wound dressing to encourage mice′s skin wounds to heal quickly.TRP was isolated utilizing the methods of water extraction,alcohol precipitation,and protein removal with trichloroacetic acid.TRP hydrogel was designed using a one-step freeze-thaw procedure,and its structure,morphology,and mechanical properties were reveal using fourier transform infrared spectroscopy(FT-IR),scanning electron microscopy(SEM),and texture analysis.In addition,the antioxidant capacity of TRP,as well as the swelling property and hemolysis rate of TRP hydrogel,were measured,and the effectiveness of TRP hydrogel as a wound dressing in promoting wound healing was investigated.According to the results,TRP hydrogel had a maximum compressive strength of 13.79 kPa and a swelling rate of 12.41.Both TRP and TRP hydrogel had a low hemolysis rate,the highest hemolysis rate of TRP was 1.29%,and the hemolysis rate of TRP6 hydrogel(TRP content:6 mg/mL)was 2.36%.TRP displayed good antioxidant activity and could effectively scavenge various free radicals.Throughout the full-thickness trauma mouse model,and H&E staining results showed that TRP hydrogel could promote the hair follicle regeneration and tissue reconstruction of mice skin wounds better.In conclusion,these results suggest that TRP hydrogel has a promising future in wound healing as a wound dressing.
作者
吴燕
寇先勇
王峣姿
刘同
刘馨月
尤祥宇
苏江涛
WU Yan;KOU Xian-yong;WANG Yao-zi;LIU Tong;LIU Xin-yue;YOU Xiang-yu;SU Jiang-tao(School of Food and Biological Engineering,Hubei University of Technology,Wuhan 430068,China)
出处
《化学试剂》
CAS
北大核心
2023年第12期26-32,共7页
Chemical Reagents
基金
国家自然科学基金青年科学基金资助项目(81703409)。
关键词
天花粉粗多糖
壳聚糖
水凝胶
抗氧化
伤口愈合
trichosanthin polysaccharides
chitosan
hydrogel
antioxidant
wound healing
作者简介
吴燕(1998-),女,湖北孝感人,硕士生,主要研究方向为传统中药新应用;通讯作者:尤祥宇,E-mail:youxiangyu@hbut.edu.cn。
