摘要
目的研究异型淋巴细胞比例联合EB病毒抗体及核酸检测在儿童传染性单核细胞增多症辅助诊断中的应用价值。方法收集2022年1—12月在平邑县人民医院就诊的37例传染性单核细胞增多症患儿的临床资料。所有患儿均进行异型淋巴细胞比例、EB病毒抗体(VCA-IgM)和EB病毒核酸(EBV-DNA)检测,记录并分析患儿的诊断结果,计算不同检测方法单独与联合应用的诊断效能。结果异型淋巴细胞比例为(16.22±3.47)%,阳性率为62.16%,VCA-IgM和EBV-DNA的阳性率分别为83.78%、91.89%;异型淋巴细胞比例检测、VCA-IgM检测和EBV-DNA检测联合应用的敏感度和准确度均高于各指标单独应用(敏感度:97.30%比43.24%、70.27%、83.78%,准确度:99.44%比84.44%、91.11%、95.00%,均P<0.05),特异度、阳性预测值与阴性预测值均明显高于异型淋巴细胞比例检测和VCA-IgM抗体检测(特异度:100.00%比95.10%、96.50%,阳性预测值:100.00%比69.57%、83.87%,阴性预测值:99.31%比86.62%、92.62%,均P<0.05)。结论异型淋巴细胞比例、VCA-IgM和EBV-DNA三项检测联合应用对于辅助诊断儿童传染性单核细胞增多症具有较高的价值。
Objective To study the application value of allotypic lymphocyte ratio detection combined with Epstein-Barr(EB)virus antibody detection and nucleic acid detection in the auxiliary diagnosis of infectious mononucleosis in children.Methods The clinical data of 37 children with infectious mononucleosis who were treated in Pingyi County People's Hospital from January to December 2022 were collectd.All the children underwent allotypic lymphocyte ratio,EB viral capsid antigen immunoglobulin M(VCA-IgM)and EB virus-DNA(EBV-DNA)detection,the diagnostic results of the patients were recorded and analyzed,and the diagnostic efficacies of different detection methods for single and combined use were calculated.Results In 37 children,the allotypic lymphocyte ratio was(16.22±3.47)%,the positive rate was 62.16%,the positive rates of VCA-IgM and EBV-DNA were 83.78%and 91.89%,respectively.The sensitivity and accuracy of the combined application of allotypic lymphocyte ratio detection,VCA-IgM detection and EBV-DNA detection were higher than those of each method used alone(sensitivity:97.30%vs.43.24%,70.27%,83.78%,accuracy:99.44%vs.84.44%,91.11%,95.00%,all P<0.05),the specificity,positive predictive value and negative predictive value were significantly higher than that of allotypic lymphocyte ratio and VCA-IgM antibody detection(specificity:100.00%vs.95.10%,96.50%,positive predictive value:100.00%vs.69.57%,83.87%,negative predictive value:99.31%vs.86.62%,92.62%,all P<0.05).Conclusion The combination of allotypic lymphocyte ratio detection,VCA-IgM detection and EBV-DNA detection is of high value in the diagnosis of infectious mononucleosis in children.
作者
乔元昊
唐阳芹
Qiao Yuanhao;Tang Yangqin(Department of Clinical Laboratory,Pingyi County People's Hospital,Linyi 273300,Shangdong,China)
出处
《实用检验医师杂志》
2023年第3期262-265,共4页
Chinese Journal of Clinical Pathologist
作者简介
通信作者:乔元昊,Email:fulljoe@163.com。
