摘要
目的 探讨血液肿瘤继发肛周脓肿患者原癌基因Src、血管内皮钙黏附素(VE-cadherin)蛋白表达情况,并分析血液肿瘤继发肛周脓肿的危险因素。方法 选取2020年5月-2021年9月上海交通大学医学院附属瑞金医院血液科收治的122例血液肿瘤患者,根据患者是否继发肛周脓肿分为肛周脓肿组40例与对照组82例。采用免疫印迹法检测Src、VE-cadherin蛋白表达量。采用酶联免疫吸附法检测血清细胞因子[白细胞介素(IL)-4、IL-6、IL-12、干扰素-γ(IFN-γ)]。分析Src、VE-cadherin蛋白表达量与血清细胞因子的相关性。分析血液肿瘤患者继发肛周脓肿的危险因素。结果 肛周脓肿组的Src蛋白表达量高于对照组,VE-cadherin蛋白表达量低于对照组(P<0.05);肛周脓肿组的血清IL-4、IL-6水平高于对照组,血清IL-12、IFN-γ水平低于对照组(P<0.05);Src蛋白表达量与血清IL-6水平呈正相关(r=0.439),VE-cadherin蛋白表达量与血清IL-6水平呈负相关(r=-0.328);有痔疮史(OR=1.763)、有糖尿病史(OR=2.787)、Src蛋白高表达(OR=1.941)是血液肿瘤患者继发肛周脓肿的危险因素,而VE-cadherin蛋白高表达(OR=0.405)是保护性因素(P<0.05)。结论 血液肿瘤继发肛周脓肿患者Src蛋白表达上调、VE-cadherin蛋白表达下调,痔疮史、糖尿病史是血液肿瘤患者继发肛周脓肿的危险因素。
OBJECTIVE To explore the expression of protooncogene Src and vascular endothelial cadherin(VE-cadherin)proteins in patients with hematologic tumors secondary to perianal abscess,and to analyze the risk factors for perianal abscess.METHODS A total of 122 patients with hematological tumor admitted to hematology department of the Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine between May 2020 and Sep 2021 were enrolled.According to whether perianal abscess occurred,they were divided into the perianal abscess group(40 cases)and control group(82 cases).The expression of Src and VE-cadherin were detected by western blot.The levels of serum cytokines,including interleukin(IL)-4,IL-6,IL-12 and interferon-(IFN-)were detected by enzyme-linked immunosorbent assay.The correlation between protein expression of Src and VEcadherin and serum cytokines was analyzed.The risk factors for perianal abscess in patients with hematologic tumor was analyzed.RESULTS The expression of Src in perianal abscess group was higher than that in the control group,while expression of VE-cadherin was lower than that in the control group(P<0.05).The levels of serum IL-4 and IL-6 in perianal abscess group were higher than those in control group,while levels of serum IL-12 and IFN-were lower than those in the control group(P<o.05).The expression of Src was positively correlated with level of serum IL-6(r=0.439),while the expression of VE-cadherin was negatively correlated with it(r=-0.328).Hemorrhoid history(OR=1.763),diabetes history(OR=2.787)and Src protein overexpression(OR=1.941)were risk factors for perianal abscess in hematopathy patients,and VE cadherin protein overexpression(OR=0.405)was a protective factor(P<0.05).CONCLUSION In patients with hematologic tumors secondary to perianal abscess,Src protein expression was up-regulated,and VE-cadherin protein expression was down-regulated.The history of hemorrhoids and diabetes were risk factors for perianal abscess in patients with hematological tumor.
作者
胡金莲
孟耀军
朱晟
朱毓蕾
周凯捷
HU Jin-ian;MENG Yao-jun;ZHU Sheng;ZHU Yu-ei;ZHOU Kai-jie(Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine,Shanghai 200025,China;不详)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2023年第15期2329-2333,共5页
Chinese Journal of Nosocomiology
基金
上海市科研基金资助项目(2020S022)。
作者简介
胡金莲(1980-),女,本科,主管护师,研究方向:血液科感染防控与护理;通讯作者:周凯捷,E-mail:small201@163.com。
