摘要
目的研究防风中5-O-甲基维斯阿米醇苷的抗炎作用及其机制。方法建立脂多糖(LPS)诱导RAW264.7小鼠单核巨噬细胞体外炎症模型,采用酶联免疫吸附测定法细胞中的白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、Toll样受体-4(TLR4)含量;采用葡聚糖硫酸钠(DSS)诱导小鼠结肠炎模型,观察5-O-甲基维斯阿米醇苷对结肠炎小鼠结肠损伤的影响,采用酶联免疫吸附测定法测定小鼠结肠组织上清液中丙二醛(MDA)和超氧化物歧化酶(SOD)水平,采用蛋白免疫印迹法(Western blot)检测结肠组织中肿瘤坏死因子(TNF-α)、IL-1β蛋白表达。结果与脂多糖组比,5-O-甲基维斯阿米醇苷各剂量组能降低RAW264.7炎症细胞中IL-1β、IL-6、TLR4含量(P<0.01),并呈现浓度依赖性;与空白组比,结肠炎模型组小鼠体质量及结肠长度降低,结肠组织有明显的病理改变。与模型组比,5-O-甲基维斯阿米醇苷各剂量组能缓解结肠炎小鼠肠炎症状和减轻肠黏膜病理损伤,降低结肠中MDA含量,增加SOD活性(P<0.01),并能下调结肠组织中的TNF-α、IL-1β蛋白表达水平(P<0.01)。结论防风中5-O-甲基维斯阿米醇苷可抑制LPS诱导小鼠RAW264.7细胞炎症,对DSS诱导小鼠结肠炎有一定的治疗作用。
Objective To study the anti-inflammation effect and mechanism of 4'-O-β-D-glucosyl-5-O-methylvisamminol in Fangfeng(Saposhnikoviae Radix).Methods The inflammatory cell model was established using LPS-induced RAW 264.7 cells.The contents of interleukin-1β(IL-1β),interleukin-6(IL-6)and Toll like receptor 4(TLR4)in the supernatant of RAW 264.7 cells inflammation model were determined by ELISA.By means of dextran sodium sulfate(DSS)-induced ulcerative colitis model,the colon mucosal lesions were observed.The levels of malondialdehyde(MDA)and superoxide dismutase(SOD)in the supernatant of mouse colon tissue were measured by ELISA and protein expressions of tumor necrosis factor-α(TNF-α)and IL-1βwere detected by Western blot.Results Compared with the LPS group,4'-O-β-D-glucosyl-5-O-methylvisamminol could significantly reduce the release of IL-1,IL-6 and TLR4 in the supernatantin with a dose-dependent manner(P<0.01).Compared with those of the blank group,the body weight and colon length of mice in colitis model group decreased and there were significant pathological changes in colon tissue.Compared with the model group,5-o-methylvisamicin can alleviate the symptoms of colitis and reduce the pathological damage of intestinal mucosa in mice with colitis,reduce the content of MDA in colon,increase the activity of SOD(P<0.01)and inhibit the protein expression levels of TNF-αand IL-1βin colon tissue(P<0.01).Conclusion 4'-O-β-D-glucosyl-5-O-methylvisamminol in Fangfeng(Saposhnikoviae Radix)can inhibit the inflammation of RAW264.7 cells induced by LPS and it has a certain therapeutic effect on DSS-induced colitis in mice.
作者
孟莉
高龙妹
刘小虎
郑威
贾天柱
关玉娟
MENG Li;GAO Longmei;LIU Xiaohu;ZHENG Wei;JIA Tianzhu;GUAN Yujuan(Liaoning Traditional Chinese Medicine Institute,Shenyang 110034,Liaoning,China;Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China)
出处
《中华中医药学刊》
CAS
北大核心
2023年第4期214-217,I0047,共5页
Chinese Archives of Traditional Chinese Medicine
基金
辽宁省教育厅科学研究项目(L201922)。
作者简介
孟莉(1980-),女,山东兖州人,研究员,博士,研究方向:中药药理与新药研发;通讯作者:关玉娟(1978-),女,辽宁沈阳人,主任医师,博士,研究方向:中医药防治呼吸系统疾病。
