摘要
以桑枝屑作为栽培基质,传统的木屑栽培基质为对照,对瓦尼桑黄Sanghuangporus vaninii菌株进行栽培,测定菌丝生长、产量、子实体多糖、黄酮和多酚含量,采用1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力、羟自由基清除能力和超氧阴离子自由基清除能力评价其活性成分的抗氧化活性,研究桑枝屑对瓦尼桑黄生长、活性成分含量和抗氧化活性的影响。结果表明,与传统木屑相比,桑枝屑对瓦尼桑黄菌丝生长、产量、活性成分含量和抗氧化活性4方面的影响均具有显著差异,随着桑枝屑含量的增加,瓦尼桑黄的菌丝生长、活性成分含量和抗氧化活性呈现逐渐上升的趋势,除菌丝生长速度均低于木屑栽培瓦尼桑黄外,活性成分和抗氧化活性均高于木屑栽培瓦尼桑黄;桑枝屑添加量为40%–60%产量最高。Pearson分析发现黄酮、多酚与DPPH自由基、超氧阴离子自由基和羟自由基清除能力均呈极显著正相关,多糖与超氧阴离子清除能力呈极显著正相关,与羟自由基清除能力呈显著正相关。综合产量、活性成分含量和抗氧化活性分析,确定适宜瓦尼桑黄菌株栽培的桑枝屑添加量宜控制在40%-60%,研究结果为瓦尼桑黄栽培基质的优化和瓦尼桑黄子实体质量评价提供了科学参考。
Mulberry sawdust was used as the cultivation substrate to cultivate Sanghuangporus vaninii.The mycelium growth,yield and the content of polysaccharides,flavonoids and polyphenols in fruiting bodies were measured.The antioxidant activity of active ingredients was evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging ability,hydroxyl free radical scavenging ability and superoxide anion free radical scavenging ability.The results showed that there were significant differences in mycelium growth,yield,the content of active components and antioxidant activity between S.vaninii cultivates with mulberry sawdust and that cultivated with traditional sawdust substrate.With the increase of the content of mulberry sawdust in cultivation substrate,the mycelium growth,the content of active components and the antioxidant activity of S.vaninii increased.Except for the mycelium growth rate,the active components and antioxidant activity of S.vaninii cultivated with mulberry sawdust were higher than those of S.vaninii cultivated with traditional ordinary sawdust.The yield of S.vaninii is the highest when cultivation substrate contains 40%–60%mulberry sawdust.Pearson analysis showed that flavonoids and polyphenols were positively correlated with DPPH free radical,superoxide anion free radical and hydroxyl radical scavenging ability,while polysaccharides were positively correlated with superoxide anion scavenging ability and hydroxyl radical scavenging ability.Comprehensive analysis of yield,the content of active ingredients and antioxidant activity indicated that the addition quantities of mulberry sawdust should be controlled in the range of 40%–60%.The research results provide a scientific reference for optimization of S.vaninii cultivation substrate.
作者
宋吉玲
陆娜
闫静
程俊文
周祖法
林佳瑶
王伟科
袁卫东
SONG Jiling;LU Na;YAN Jing;CHENG Junwen;ZHOU Zufa;LIN Jiayao;WANG Weike;YUAN Weidong(Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,Zhejiang,China;Zhejiang Academy of Forestry,Hangzhou 310023,Zhejiang,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2023年第4期949-960,共12页
Mycosystema
基金
现代农业产业技术体系建设专项资金(CARS-20)
国家自然科学基金(31501815)
浙江省科技厅项目(2021C02073,2019F1065-2)
杭州市财政项目(2021HNCT-01)。
关键词
瓦尼桑黄
桑枝屑
多糖
黄酮
多酚
抗氧化活性
S.vaninii
mulberry sawdust
polysaccharide
flavonoid
polyphenol
antioxidant activities
作者简介
SONG Jiling,ORCID:0000-0003-4858-4319;Corresponding authors:王伟科,杭州市农业科学研究院蔬菜研究所高级农艺师,国家食用菌产业技术体系综合试验站成员,长期从事秀珍菇、桑黄等食药用菌新品种选育及配套栽培技术研究。E-mail:akeak@126.com,ORCID:0000-0002-2228-6519;Corresponding authors:袁卫东,杭州市农业科学研究院正高级农艺师,杭州市政府特殊津贴、浙江省优秀科技特派员和浙江省食用菌行业突出贡献奖获得者。现任国家食用菌产业技术体系团队成员、浙江省食用菌产业创新团队专家、浙江省农业技术带头人,中国菌物学会黑木耳分会理事等职,从事食用菌新品种选育与高效栽培技术研究。E-mail:ywd0507@126.com,ORCID:0000-0002-3597-6981。
