摘要
目的:研究丹参素对人胃腺癌AGS细胞凋亡的影响及其机制。方法:将人胃腺癌AGS细胞在体外培养至其生长良好后用不同浓度的丹参素处理AGS细胞24 h、48 h,用CCK8、流式细胞仪、Western blotting法检测丹参素对AGS细胞增殖抑制、凋亡、周期和蛋白表达的影响。结果:与空白组比较,丹参素作用于AGS细胞后对其生长起着抑制作用(P<0.05),具有时间和浓度依赖关系;且可促进细胞凋亡(P<0.05),阻滞细胞于G2/M期(P<0.05);促进p53、Cytochrome C、Bax蛋白表达(P<0.05),抑制BCL-2蛋白表达(P<0.05)。结论:丹参素能够抑制胃癌AGS细胞的生长,促进AGS细胞凋亡,其机制与丹参素阻滞细胞于G2/M期、启动线粒体凋亡有关。
Objective:To study the effect of Danshensu on the apoptosis of human gastric adenocarcinoma AGS cells and its mechanism.Methods:Human gastric adenocarcinoma cell lines AGS were cultured in vitro until it grew well.AGS cells were treated with different concentrations of Danshensu for 24 h and 48 h.The effects of danshensu on AGS cell proliferation inhibition,apoptosis,cycle and protein expression were detected using CCK8,flow cytometry and Western blotting methods.Result:Compared with the blank group,Danshensu inhibited the growth of AGS cells after acting on them(P<0.05),with a time-dependent and concentration-dependent relationship.It promoted cell apoptosis(P<0.05)and arrested cells in G2/M phase(P<0.05).It promoted the protein expression of p53,Cytochrome C and Bax(P<0.05),and inhibited the protein expression of BCL-2(P<0.05).Conclusion:Danshensu can inhibit the growth of gastric cancer AGS cells and promote the apoptosis of AGS cells.The mechanism is related to the arrest of cells in G2/M phase and the initiation of mitochondrial apoptosis.
作者
邓帅
周桂香
郭小峰
谭宝
DENG Shuai;ZHOU Guixiang;GUO Xiaofeng;TAN Bao(Shanxi University of Traditional Chinese Medicine,Taiyuan Shanxi 030024,China;Shanxi Hospital of Traditional Chinese Medicine,Taiyuan Shanxi 030000,China)
出处
《中医药导报》
2023年第4期1-4,23,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
山西省科技厅重大基础研发规划专项(201903D321215)。
关键词
丹参素
胃癌细胞
细胞凋亡
细胞周期
机制研究
Danshensu
gastric cancer cells
cell apoptosis
cell cycle
mechanism research
作者简介
通信作者:谭宝,E-mail:tanbaopeter@sina.com。
