摘要
目的通过网络药理学探索路氏润燥汤治疗干燥综合征的可能作用机制,并通过动物实验进行验证。方法通过TCMSP数据库检索收集路氏润燥汤方中各药物生物信息,通过Genecard数据库检索收集干燥综合征的靶标蛋白,将药物和疾病的靶点信息进行交集,获得交集信息。将信息导入STRING平台获得PPI网络图,将筛选的得到的药物成分与靶点信息通过Cytospace软件构建“成分-靶点”网络图,利用DAVID数据库进行GO富集分析和KEGG通路分析。通过动物实验对网络药理学预测出的通路信息进行验证。结果路氏润燥汤主要通过影响肿瘤坏死因子、细胞因子,肿瘤坏死因子信号通路、IL-17信号通路等信号通路来治疗干燥综合征。结论路氏润燥汤是通过多靶点、多通路来治疗干燥综合征,网络药理学可以对路氏润燥汤进行较为全面的预测,有助于临床实验研究。
Objective To explore the possible mechanism of Lushi Runzao Decoction in treating Sjogren’s syndrome through network pharmacology,and verify it through animal experiments.Methods The biological information of each drug in Lushi Runzao Decoction was retrieved and collected by TCMSP database,and the target protein of Sjogren’s syndrome was retrieved and collected by Genecard database.The target information of drugs and diseases was intersected to obtain the intersection information.We imported the information into STRING platform to obtain PPI network diagram,constructed the"component-target"network diagram with the screened drug components and targeted information through Cytospace software,and used DAVID database to analyze GO enrichment and KEGG pathway.The pathway information predicted by network pharmacology was verified by animal experiments.Results Lushi Runzao Decoction mainly treated Sjogren’s syndrome by influencing tumor necrosis factor,cytokines,chemokines,tumor necrosis factor signal pathway,IL-17 signal pathway and AGE-RAGE signal pathway.Conclusion Lushi Runzao Decoction can treat Sjogren’s syndrome through multi-target and multi-channel.Network pharmacology can make a comprehensive prediction for Lushi Runzao Decoction,which is helpful for clinical research.
作者
庞枫韬
李克嵩
张依
夏聪敏
周新尧
唐晓颇
Pang Fengtao;Li Kesong;Zhang Yi;Xia Congmin;Zhou Xinyao;Tang Xiaopo(Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2022年第9期3406-3418,共13页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会青年科学基金项目(81804069):从白介素17-水通道蛋白-血清AQPs抗体探讨“针刺润燥”治疗干燥综合征增效,负责人:周新尧
关键词
路氏润燥汤
干燥综合征
网络药理学
实验验证
分子机制
Lushi Runzao Decoction
Sjogren’s syndrome
Network pharmacological
Experimental verification
Molecular mechanism
作者简介
通讯作者:唐晓颇,博士后,中国中医科学院广安门医院风湿科主任医师,博士生导师,主要研究方向:中医药防治风湿病。
