摘要
目的分析血清长链非编码RNA(lncRNA)X非活性特异性转录本(XIST)水平与老年膝关节骨性关节炎(KOA)患者病情严重程度及炎性因子水平的相关性。方法选择2019年3月至2021年4月确诊的96例KOA患者为研究对象(研究组),同期健康体检者100例作为对照(对照组),实时荧光定量PCR(RT-qPCR)法检测血清中XIST水平,酶联免疫吸附(ELISA)检测血清中白介素(IL)-1β、肿瘤坏死因子-α(TNF-α)、白介素(IL-6)水平。根据X线片评估,参考Kellgren-Lawrence(K-L)分级标准对所有受试者病情严重程度进行分级;Spearman分析KOA患者病情严重程度与血清中XIST、IL-1β、TNF-α、IL-6水平关系;Pearson分析KOA患者血清中XIST与IL-1β、TNF-α、IL-6水平关系。结果与对照组比较,研究组血清中XIST、IL-1β、TNF-α、IL-6水平升高(P<0.05)。K-L分级判定结果:对照组均为0级,研究组Ⅰ级19例、Ⅱ级24例、Ⅲ级36例、Ⅳ级17例。Spearman分析发现KOA患者血清中XIST、IL-1β、TNF-α、IL-6水平与病情严重程度呈正相关(r值分别为0.784、0.791、0.657、0.703,P<0.05);Pearson分析发现KOA患者血清中XIST水平与IL-1β、TNF-α、IL-6水平均呈正相关(r值分别为0.635、0.525、0.414,P<0.05)。结论老年KOA患者血清中XIST水平升高,且其与病情严重程度、炎性因子水平关系密切,可能对于指导临床治疗老年KOA患者具有一定意义。
Objective To analyze the correlation of serum long non-coding RNA(lncRNA)X-inactive-specific transcript(XIST)level with the severity and expression levels of inflammatory factors in elderly patients with knee osteoarthritis(KOA).Methods Ninety-six KOA patients diagnosed in our hospital from March 2019 to April 2021 were included in the study group,and 100 healthy subjects were included in the control group.The relative level of XIST was measured by real-time quantitative PCR(RT-qPCR).Serum levels of interleukin(IL)-1β,tumor necrosis factor-α(TNF-α)and IL-6 were measured by enzyme-linked immunosorbent assay(ELISA).According to the evaluation of X-ray films,the severity of KOA was graded based on the Kellgren-Lawrence(K-L)grading standard.Spearman’s rank correlation test was performed to analyze the correlation between the severity of KOA and expression levels of XIST,IL-1β,TNF-αand IL-6.Pearson correlation test was performed to analyze the correlation between the relative level of XIST and serum levels of IL-1β,TNF-α,and IL-6 in KOA patients.Results Compared with those of the control group,serum levels of XIST,IL-1β,TNF-αand IL-6 in the study group were significantly higher(P<0.05).Based on the K-L classification,all patients in the control group were grade 0.There were 19,24,36 and 17 patients of grade I-IV in the study group,respectively.Spearman analysis showed that expression levels of XIST,IL-1β,TNF-αand IL-6 were positively correlated with the severity of KOA(r=0.784,0.791,0.657,and 0.703,respectively;all P<0.05).Pearson analysis displayed XIST level in KOA patients was positively correlated with serum levels of expression levels of IL-1β,TNF-αand IL-6(r=0.635,0.525 and 0.414,respectively,all P<0.05).Conclusion XIST is upregulated in elderly patients with KOA,which is closely correlated with the severity of KOA and serum levels of inflammatory factors.Our results provide references for guiding the clinical treatment of elderly patients with KOA.
作者
吕根兵
李军
LV Genbing;LI Jun(Department of Orthopedics,Tongchuan City Maternal and Child Health Care,Shaanxi,Tongchuan 727007,China)
出处
《河北医药》
CAS
2023年第5期671-674,共4页
Hebei Medical Journal
基金
国家自然科学基金面上项目(编号:8167090690)。
