摘要
目的探讨血清核心蛋白聚糖(Decorin)、可溶性Axl(sAxl)和细胞因子样蛋白1(CYTL1)水平与膝骨关节炎患者Kellgren-Lawrence(K-L)分级的关系。方法选取2021年8月至2023年5月该院收治的膝骨关节炎患者328例作为研究组,另选取同期体检健康者328例作为对照组。采用酶联免疫吸附试验检测血清Decorin、sAxl和CYTL1水平,西安大略和麦克马斯特大学骨性关节炎调查量表(WOMAC)评估患者临床症状。采用Spearman法分析血清Decorin、sAxl和CYTL1与K-L分级和WOMAC评分的相关性,Logistic回归分析膝骨关节炎的影响因素,绘制受试者工作特征(ROC)曲线分析血清Decorin、sAxl和CYTL1对膝骨关节炎Ⅰ~Ⅱ级、Ⅲ~Ⅳ级的诊断价值。结果研究组血清Decorin、sAxl水平显著高于对照组,CYTL1显著低于对照组,差异均有统计学意义(P<0.05)。K-L分级Ⅰ级、Ⅱ级、Ⅲ级、Ⅳ级血清Decorin、sAxl水平和WOMAC评分依次升高(P<0.05),CYTL1水平依次降低(P<0.05)。Spearman相关性分析显示,血清Decorin、sAxl与K-L分级和WOMAC评分均呈正相关(P<0.05),CYTL1与K-L分级和WOMAC评分呈负相关(P<0.05)。Decorin、sAxl高水平、CYTL1低水平为膝骨关节炎的危险因素(P<0.05)。血清Decorin、sAxl和CYTL1水平对膝骨关节炎Ⅰ~Ⅱ级、Ⅲ~Ⅳ级有一定诊断价值。结论血清Decorin、sAxl和CYTL1水平与膝骨关节炎K-L分级密切相关,均可用于膝骨关节炎Ⅰ~Ⅱ级、Ⅲ~Ⅳ级的辅助诊断。
Objective To explore the relationship between serum levels of decorin,soluble Axl(sAxl),cytokine-like protein 1(CYTL1)and Kellgren-Lawrence(K-L)grading in patients with knee osteoarthritis.Methods From August 2021 to May 2023,328 patients with knee osteoarthritis admitted to the hospital were selected as the research group,and 328 healthy controls during the same period were selected as the control group.Serum Decorin,sAxl and CYTL1 levels were detected by enzyme-linked immunosorbent assay.Western Ontario and McMaster University Osteoarthritis Questionnaire(WOMAC)was used to assess patients′clinical symptoms.Spearman method was used to analyze the correlation between serum Decorin,sAxl,CYTL1 and K-L grading,as well as WOMAC score.Logistic regression was used to analyze the influencing factors of knee osteoarthritis.ROC curve was drawn to analyze the diagnostic value of serum Decorin,sAxl and CYTL1 in gradeⅠtoⅡandⅢtoⅣof knee osteoarthritis.Results Serum Decorin and sAxl levels in the study group were significantly higher than those in the control group(P<0.05),and CYTL1 levels were significantly lower than those in the control group,with statistical significance(P<0.05).The levels of serum Decorin,sAxl and WOMAC scores in K-L gradesⅠ,Ⅱ,ⅢandⅣwere increased successively(P<0.05),while the levels of CYTL1 were decreased successively(P<0.05).Spearman correlation analysis showed that serum Decorin and sAxl were positively correlated with K-L grading and WOMAC score(P<0.05),and CYTL1 was negatively correlated with K-L grading and WOMAC score(P<0.05).High level of Decorin,sAxl and low level of CYTL1 were risk factors for knee osteoarthritis(P<0.05).The levels of serum Decorin,sAxl and CYTL1 had certain diagnostic value for gradeⅠtoⅡand gradeⅢtoⅣof knee osteoarthritis.Conclusion Serum Decorin,sAxl and CYTL1 levels are closely related to K-L grade of knee osteoarthritis,and can be used for auxiliary diagnosis of gradeⅠtoⅡand gradeⅢtoⅣof knee osteoarthritis.
作者
胡国新
李太极
刘春晖
HU Guoxin;LI Taiji;LIU Chunhui(Pain Department,Qinhuangdao Traditional Chinese Medicine Hospital,Qinhuangdao,Hebei 066000,China;Second Department of Orthopedics,Qinhuangdao Traditional Chinese Medicine Hospital,Qinhuangdao,Hebei 066000,China)
出处
《国际检验医学杂志》
CAS
2024年第17期2132-2135,2140,共5页
International Journal of Laboratory Medicine
基金
秦皇岛市科学技术研究与发展计划(202301A057)。
作者简介
胡国新,男,主治医师,主要从事慢性疼痛诊疗及研究;通信作者:刘春晖,E-mail:lchflyer@163.com。
