摘要
文章建立了超高效液相色谱-串联质谱(UHPLC-MS/MS)法同时测定水产品中18种卡因类麻醉剂的含量。样品采用磷酸盐缓冲溶液提取,混合阳离子固相萃取柱(MCX)净化,乙腈和0.3%甲酸水为流动相,经HSS T3色谱柱分离,采用UHPLC-MS/MS进行分析。方法学验证表明,18种化合物的线性关系良好,相关系数(R^(2))均大于0.999;检出限为0.2~0.4μg/kg,定量限在0.7~1.3μg/kg之间;以鲈鱼、明虾、螃蟹为基质,进行3个不同加标水平试验(n=6),18种化合物的平均回收率在84.7%~108.7%之间,相对标准偏差RSD在1.6%~7.5%之间。该方法具有较高的灵敏度、准确度以及重现性,适用于水产品中18种卡因类残留的测定。
A method was established for the simultaneous determination of 18 kinds of caine anesthetic in aquatic products by ultra-high performance liquid chromatography coupled with tandem mass spectrometry(UHPLC-MS/MS). The samples were extracted by phosphate buffer solution, purified by mixed cationic solid phase extraction(MCX) column, separated by HSS T3 column with acetonitrile and 0.3% formic acid water as mobile phases and then analysis by UHPLC-MS/MS. The correlation coefficients(R^(2)) of the 18 compounds were all greater than 0.999. The limit of detection was between 0.2 μg/kg and 0.4 μg/kg, the limit of quantification was between 0.7 μg/kg and 1.3 μg/kg. In sea bass,prawns, crabs samples, the mean recoveries obtained at three concentration levels were between 84.7%and 108.7%, with relative standard deviations(RSDs) between 1.6% and 7.5%. The method has high sensitivity, accuracy and reproducibility, and is suitable for the determination of 18 kinds of caine residues in aquatic products.
作者
吴少明
陈言凯
刘文菁
欧阳立群
苏晓明
林钦
戴明
WU Shaoming;CHEN Yankai;LIU Wenjing;OUYANG Liqun;SU Xiaoming;LIN Qin;DAI Ming(Fujian Inspection and Research Institute for Product Quality,National Quality Supervision and Testing Center for Processed Food,Fuzhou 350002,China)
出处
《食品科技》
CAS
北大核心
2023年第1期285-291,共7页
Food Science and Technology
基金
国家市场监督管理总局科技计划项目(2021MK054)。
作者简介
吴少明(1986-),男,江西人,硕士,研究方向为食品安全;通信作者:戴明。
