摘要
[目的]建立一种同时测定茶叶中毒菌酚、戊硝酚、消螨酚和特乐酚4种农药残留的分析方法。[方法]将茶叶样品经水涡旋浸泡、酸性乙腈提取、改进的QuEChERS法净化后,再采用超高效液相色谱-串联质谱检测,基质匹配标准溶液外标法定量。[结果]4种农药在各自浓度范围内线性良好,相关系数(r)均大于0.99,检出限(LOD)和定量限(LOQ)分别为0.05~0.17μg/kg和10μg/kg;在空白茶叶中添加10、20、100μg/kg 3个质量分数水平下具有良好的回收率和精密度,回收率为72.7%~104.1%,RSD为2.7%~8.8%。[结论]该方法可用于茶叶中4种农药残留的检测。
[Aims]This study aims to develop an analytical method for the simultaneous detection of hexachlorophene,dinosam,dinex and dinoterb pesticides in tea.[Methods]The tea samples were wetted by water and extracted with acetic acid-acetonitrile,and the extract was purified by improved QuEChERS pretreatment.Quantitative detection was performed by ultra performance liquid chromatography-tandem mass spectrometry,the results were quantified by the external standard method.[Results]Under the optimized conditions,the results showed that the 4 pesticides had good linearity within the range of 1-50μg/L,with correlation coefficients(r)higher than 0.99.The average recoveries ranged from 72.7%to 104.1%,and the relative standard deviation(RSD)was 2.7%-8.8%at three levels(10,20 and 100μg/kg).The LODs were from 0.05 to 0.17μg/kg and LOQs were 10μg/kg.[Conclusions]This method was used for the detection of four pesticide residues in tea.
作者
郑坤明
林瑶
吴晓萍
陈建南
张钰萍
ZHENG Kun-ming;LIN Yao;WU Xiao-ping;CHEN Jian-nan;ZHANG Yu-ping(Fujian CCIC-Fairreach Food Safety Testing Co.,Ltd.,Fuzhou 350028,China;Research and Development Center for Fine Chemicals,State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering,Key Laboratory of Green Pesticide and Agricultural Bioengineering,Ministry of Education,Guizhou University,Guiyang 550025,China)
出处
《农药》
CAS
CSCD
北大核心
2022年第10期743-746,共4页
Agrochemicals
基金
国家自然科学基金(21667007)
关键词
QUECHERS
超高效液相色谱-串联质谱
茶叶
农药残留
QuEChERS
ultra performance liquid chromatography-tandem mass spectrometry
tea
pesticide residues
作者简介
郑坤明(1992—),男,福建泉州人,工程师,主要从事农药残留分析。E-mail:18198639783@163.com。
