摘要
采用超声法提取龙眼叶总黄酮,运用紫外分光光度法测定龙眼叶总黄酮含量,并检测和对比不同浓度的龙眼叶总黄酮和阳性药阿卡波糖对α-葡萄糖苷酶的抑制率。研究发现,提取的龙眼叶总黄酮含量为0.3753 mg·g^(-1),龙眼叶总黄酮浓度为0.25 mg·mL^(-1)与阿卡波糖浓度为0.3μg·mL^(-1)时α-葡萄糖苷酶抑制率同为65.09%,说明在此浓度下,龙眼叶总黄酮的降血糖能力与阿卡波糖相近。结果表明,龙眼叶中总黄酮具有一定降血糖能力,同时该结果可为降血糖研究路上开辟新途径奠定基础。
The total flavonoids of longan leaves was extracted by ultrasonic method, and the content of total flavonoids of longan leaves was determined by ultraviolet spectrophotometry. The inhibitory rate of total flavonoids from longan leaves on α-glucosidase with different concentrations and the inhibitory rate of positive drug acarbose on α-glucosidase was detected, then their inhibitory rate was compared. The final measure showed that the content of total flavonoid in longan leaves sample was 0.3753 mg·g^(-1). The inhibition rate of α-glucosidase was 65.09% when the concentration of total flavonoids in longan leaves was 0.25 mg·mL^(-1)and the concentration of acarbose was 0.3 μg·mL^(-1). The hypoglycemic activity of total flavonoids in longan leaves was similar to that of acarbose at this concecntration, and the results showed that the total flavonoids of longan leaves had a certain inhibitory effect on α-glucosidase. The study will provide experimental datas for medicinal value of longan leaves and may be used potentially as a good source for hypoglycemic drugs.
作者
黄淑婷
巫秋霖
莫漫莉
张李江
王华鸣
姜涛
HUANG Shu-ting;WU Qiu-lin;MO Man-li;ZHANG Li-jiang;WANG Hua-ming;JIANG Tao(School of Pharmacy Food Science,Zhuhai College of Science and Technology,Guangdong Zhuhai 519041,China)
出处
《广州化工》
CAS
2022年第24期50-52,共3页
GuangZhou Chemical Industry
基金
广东省大学生创新创业训练计划项目(No:s202013684009)。
关键词
龙眼叶
总黄酮
Α-葡萄糖苷酶
酶活力测定
降血糖
longan leaves
total flavonoids
α-glucosidase
determination of enzyme activity
hypoglycemic
作者简介
第一作者:黄淑婷(2000-),女,制药工程专业本科生;通讯作者:姜涛(1982-),男,讲师,主要从事生物化学研究。
