摘要
病毒性肺炎是一种传染性极强的呼吸系统疾病,病程发展急骤且近年来发病率处于上升趋势,因此复制出更加符合中西医临床病证特点的动物模型尤为重要。本文通过对大量文献的归纳整理,对现有模型的方法、机制及原理进行总结,依据拟定的中西医临床诊断标准对各种模型的中西医吻合度进行评价。结果显示高脂饲料+病毒滴鼻+游泳30 min引起的湿热证型病毒性肺炎模型中西医临床吻合度较高,该模型动物表现的发热、咽痛、咳嗽、鼻塞流涕、呼吸困难等症状与人类病毒性肺炎临床特征较符合,且肺组织病理变化明显,气道上皮细胞脱落、支气管黏膜下及气管壁周围有大量炎性细胞浸润,高度符合病毒性肺炎湿热证型。本文通过分析病毒性肺炎各造模方法的优缺点,对改进完善其动物模型提出建议;同时为深入研究其发病机制及诊疗方法提供思路,以促进病毒性肺炎的临床研究。
Viral pneumonia is a highly infectious respiratory disease.The disease course develops rapidly and the incidence rate is increasing.Therefore,it is important to establish an animal model that corresponds to the clinical characteristics of traditional Chinese and Western medicines.This review summarizes the method,mechanisms and principles of the existing models by summarizing a large number of studies.In accordance with the proposed clinical diagnostic criteria of traditional Chinese and Western medicines,this study evaluated the anastomosis degree of traditional Chinese and Western medicines of various models.The result showed that the model of viral pneumonia with damp heat syndrome caused by a high fat diet,virus nasal drip,and swimming for 30 min had a high degree of clinical coincidence between traditional Chinese and Western medicines.The symptoms of fever,sore throat,cough,nasal congestion,runny nose,and dyspnea of the model animals are more consistent with the clinical characteristics of human viral pneumonia,and the pathological changes of lung tissue were obvious.There was a large number of inflammatory cells under the bronchial mucosa and around the tracheal wall,which was highly consistent with the damp heat syndrome type of viral pneumonia.By analyzing the advantages and disadvantages of each modeling method of viral pneumonia,this study provides suggestions on improving the animal model and ideas for in-depth study of the pathogenesis,diagnosis and treatment method to promote clinical research of viral pneumonia.
作者
狄培琰
张双丽
康乐
苗明三
DI Peiyan;ZHANG Shuangli;KANG Le;MIAO Mingsan(Henan University of Chinese Medicine,Zhengzhou 450046,China)
出处
《中国比较医学杂志》
CAS
北大核心
2022年第10期123-129,共7页
Chinese Journal of Comparative Medicine
基金
国家中医药管理局标准化项目(GZY-FJS-2020-219)
河南省重大公益专项(201300310100)
河南省中医药科学研究专项课题(20-21ZYZD02)。
关键词
病毒性肺炎
病证结合
动物模型
中西医
诊断标准
viral pneumonia
combination of disease and syndrome
animal model
traditional Chinese and Western medicines
diagnostic criteria
作者简介
狄培琰(1996-),女,硕士研究生,研究方向:中药学。E-mail:15136370813@163.com;通信作者:苗明三(1965-),男,教授,博士,研究方向:中药药理学。E-mail:miaomingsan@163.com。
