摘要
旨在研究单独添加屎肠球菌和枯草芽孢杆菌及二者混合添加对乳鸽生长性能、屠宰性能、免疫功能的影响,从而探究益生菌对乳鸽生长的调控机制。试验选取240只12日龄、体重接近的健康白羽王鸽,随机分成4组,分别为对照组、屎肠球菌组、枯草芽孢杆菌组、混合组,每组6个重复,每个重复10只乳鸽,试验期16 d。试验期结束时,每个重复随机选取2只乳鸽(共48只乳鸽)称重,测定其血清免疫指标、肌肉品质、肝组织抗氧化指标和生长相关基因表达量等。试验结果表明:1)与对照组相比,屎肠球菌组显著提高了乳鸽的平均日增重(P<0.05),且显著高于其余各组(P<0.05),而枯草芽孢杆菌组显著降低了乳鸽的平均日增重(P<0.05);2)与对照组相比,3个处理组对乳鸽的屠宰率、半净膛率、全净膛率、腿肌率、胸肌率、腹脂率均无显著影响(P>0.05),屎肠球菌组和混合组显著提高了乳鸽胸肌亮度(P<0.05),混合组还显著提高了乳鸽胸肌红度(P<0.05);3)与对照组相比,屎肠球菌组和混合组能显著提高乳鸽血清中IgG含量(P<0.05),混合组能显著提高乳鸽胸腺指数和法氏囊指数(P<0.05),除屎肠球菌组GLB显著高于对照组(P<0.05)外,3个处理组对乳鸽的其余血清生化指标均无显著影响(P>0.05);4)与对照组相比,屎肠球菌组超氧化物歧化酶(SOD)活性显著提高(P<0.05),混合组谷胱甘肽过氧化物酶(GSH-Px)活性显著提高(P<0.05);5)3个处理组肝组织的GH、IGF-1基因mRNA表达水平均显著高于对照组(P<0.05),枯草芽孢杆菌组GHR基因mRNA表达水平显著高于对照组(P<0.05)。综上所述,屎肠球菌可显著提高乳鸽平均日增重,而枯草芽孢杆菌显著降低了乳鸽的平均日增重;屎肠球菌能改善乳鸽肌肉品质,提高乳鸽抗氧化性能和免疫功能,从而促进乳鸽生长。
This study was designed to investigate the effects of Enterococcus faecium and Bacillus subtilis addition alone and mixed addition on growth performance,carcass traits and immune function of squabs,in order to explore the regulatory mechanism of probiotics on squabs growth.Two hundred and forty squabs at 12 days of age with similar body weight were selected and randomly divided into 4 groups,including the control group,Enterococcus faecium group,Bacillus subtilis group and mixed group,with 6 replicates per group and 10 squabs per replicate.The trial lasted for 16 days.At the end of the experiment,two squabs from each replicate(a total of 48 pigeons) were randomly selected.Serum immune indices,muscle quality,liver antioxidant indices and growth-related genes expression levels were determined.The results showed that:1) Compared with the control group,the average daily gain of squabs in the Enterococcus faecium group was significantly induced and was significantly higher than that of the other groups(P0.05);Compared with control group,the brightness of breast muscle in Enterococcus faecium group and mixed group were significantly increased(P0.05);4) Compared with control group,the activity of superoxide dismutase(SOD) in Enterococcus faecium group was significantly higher(P<0.05),the glutathione peroxidase(GSH-Px) activity in mixed group was significantly higher(P<0.05);5) The mRNA expression of GH and IGF-1 in liver of three experimental groups were significantly higher than those in the control group(P<0.05),and the mRNA expression of GHR in liver of Bacillus subtilis group as significantly higher than that in the control group(P<0.05).In conclusion,Enterococcus faecium can significantly increased the average daily gain of squabs,while Bacillus subtilis had the oppsite effect;Enterococcus faecium can improve the meat quality,enhance the immune capacity and antioxidant capacity,thus promote the growth of squabs.
作者
韩鹏敏
张然
边世雄
李云雷
倪爱心
王园美
宗云鹤
赵金蒙
袁经纬
孙研研
李建慧
陈继兰
麻慧
HAN Pengmin;ZHANG Ran;BIAN Shixiong;LI Yunlei;NI Aixin;WANG Yuanmei;ZONG Yunhe;ZHAO Jinmeng;YUAN Jingwei;SUN Yanyan;LI Jianhui;CHEN Jilan;MA Hui(Key Laboratory of Animal(Poultry)Genetics Breeding and Reproduction of Ministry of Agriculture and Rural Affairs,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Animal Science,Shanxi Agricultural University,Taigu 030801,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2022年第11期3880-3891,共12页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
现代农业产业技术体系北京市家禽创新团队项目(BAIC04-2021)
国家自然科学基金青年基金项目(31802058)
中国农业科学院科技创新工程项目(ASTIP-IAS04)。
关键词
屎肠球菌
枯草芽孢杆菌
乳鸽
生长性能
免疫
Enterococcus faecium
Bacillus subtilis
squabs
growth performance
immunity
作者简介
韩鹏敏(1997-),女,山西汾阳人,硕士生,主要从事家禽遗传育种与繁育研究,E-mail:604307489@qq.com;通信作者:麻慧,主要从事家禽遗传育种与繁殖研究,E-mail:caumah@163.com;通信作者:陈继兰,主要从事家禽遗传育种与繁殖研究,E-mail:chen.jilan@163.com。
