摘要
目的探讨rhGH对肝硬化大鼠肝部分切除术后GH/IGF-1轴的影响。方法6只正常大鼠作为正常对照组,24只肝硬化大鼠随机分为术前组6只,肝部分切除术后1d组6只,肝部分切除后行PN5d组6只,肝部分切除术后行rhGH+PN5d组6只。测大鼠肝功能、血糖及血清GH、IGF-1、IGFBP-3水平,用RT-PCR法检测肝ALBmRNA、IGF-1mRNA、IGFBP-3mRNA的表达。肝组织行Ki67免疫组化染色。结果与PN组比较,rhGH+PN组血清ALP显著下降(P<0·05),血清ALB显著升高(P<0·05),血糖显著升高(P<0·05),血清GH、IGF-1、IGFBP-3水平显著升高(P<0·05),肝ALBmRNA、IGF-1mRNA表达水平显著升高(P<0·05),肝Ki67指数也显著升高(P<0·05)。且血清GH水平与血糖呈正相关(P<0·05),血清IGF-1、IGFBP-3水平与血清AST、ALT、ALP呈负相关(P<0·05),与血清ALB量正相关(P<0·05)。结论rhGH可以改善肝硬化大鼠肝部分切除术后GH/IGF-1轴,血清IGF-1、IGFBP-3水平有助于判断营养支持的效果。
Objective To investigate the effects of recombinant human growth hormone (rhGH) on growth hormone/insulin like growth factor-1 (GH/IGF) axis after partial hepatectomy in rats with liver cirrhosis. Methods Twenty-four rats with liver cirrhosis were randomized into the pre-operation group (n=6), postoperative 1-d group (n=6), postoperative PN group (n=6) and postoperative rhGH+PN group (n=6). Meanwhile, 6 normal rats were employed to serve as the controls. Liver function, blood glucose and serum GH, IGF-1, IGFBP-3 levels were determined. The levels of ALB MRNA, IGF-1 mRNA and IGFBP-3 mRNA were determined by RT-PCR. Liver Ki67 immunohistochemistry was also conducted. Results Compared with PN group, the serum level of ALP was significantly decreased (P<0.05) while serum level of ALB, blood glucose level, serum level of GH, IGF-1, IGFBP-3, the levels of ALB mRNA and IGF-1 mRNA in the liver and the liver Ki67 index were markedly increased (P<0.05). The serum level of GH was positively correlated to blood glucose (P<0.05) while the serum level of IGF-1 and IGFBP-3 were negatively correlated with those of AST, ALT and ALP (P<0.05) but positively correlated to that of ALB (P<0.05). Conclusions rhGH can improve GH/IGF-1 axis after partial hepatectomy in rats with liver cirrhosis. Measurement of serum IGF-1 and IGFBP-3 might be helpful for estimation of the effect of nutritional support after operation.
出处
《中华肝胆外科杂志》
CAS
CSCD
2005年第4期257-260,共4页
Chinese Journal of Hepatobiliary Surgery
