摘要
目的建立高效液相色谱法测定桑白皮中桑酮G、桑酮H的含量,对不同产地桑白皮含量进行分析.方法采用Agilent Eclipse XDB C18(4.6mm×250mm,5μm)色谱柱;以乙腈(A)-0.1%甲酸溶液(B)为流动相.柱温30℃,检测波长265nm,进样体积10μl.结果桑酮G在浓度0.13-3.44μg,y=2×10^(6)x+4.315×103线性关系良好,R1=0.9999,桑酮H在浓度0.13-3.36μg,y=8.249×10^(5)x-6.281×10^(3)线性关系良好,R2=0.9999,桑酮G回收率在96.5%-98.0%、桑酮H回收率在95.7%-99.2%;重复性、稳定性、精密度RSD值均在2%以下.结论本方法操作简便、准确,可作为桑白皮中桑酮G、桑酮H含量测定的检测方法,含量结果显示不同产地桑白皮中桑酮G、桑酮H二者之和含量在2.13%-19.92%,表明不同产地差异较大.
Objective To determine Kuwanon G and Kuwanon H in mori cortex by HPLC.Teh contents of mulberry bark from different habitats were analyzed.Methods Using Agilent eclipse XdB C18(4.6mm×250mm,5μm)Chromatographic column;acetonitrile(a)-0.1%formic acid water(b)was used as the mobile phase.The column temperature is 30℃,the detection wavelength is 265nm,and the injection volume is 10μl.Results The concentration of Kuwanon G is 0.13-3.44μg,y=2×10^(6)x+4.315×10^(3),R1=0.9999,the concentration of Kuwanon H is 0.13-3.36μg,y=8.249×10^(5)x-6.281×10^(3),R^(2)=0.9999,the recoveries of Kuwanon G and Kuwanon H were 96.5%-98.0%and 95.7%-99.2%.Conclusion This method is simple and accurate,it can be used for the dertmeination of the content of Kuwanon G an d Kuwanon H in mori cortex.The content results show that the sum of Kuwanon G and Kuwanon H in mori cortex from different habitats is 2.13%-19.92%,indicating that there are great differences.
作者
刘萱
秦雯
李铮
张宪
杜小伟
Liu Xuan;Qin Wen;Li Zheng;Zhang Xian;Du Xiaowei(Beijing Institute For Drug Control(Beijing Center For Vaccine Control),NMPA Key Laboratory for quality evaluation of Chinese patent medicine,Beijing Key Laboratory of Analysis and Evaluation on Chinese Medicine,Beijing 102206,China;Beijing City University,Beijing 100083,China)
出处
《首都食品与医药》
2022年第18期121-124,共4页
Capital Food Medicine
