摘要
目的:建立同时测定桑巴皮中桑酮G、桑酮H和桑辛素含量的高效液相色谱法,采用响应曲面法对该3个成分的提取工艺进行优化。方法:分别以桑酮G、桑酮H、桑辛素的提取含量作为响应值,以乙醇体积分数、提取温度、提取时间为主要考察因素,在单因素试验基础上采用中心组合设计试验,确定3个成分的最佳提取工艺。结果:同时提取桑白皮中桑酮G、桑酮H和桑辛素的最佳工艺为乙醇体积分数68%,提取温度74℃,提取时间110 min,液料比14∶1,该工艺桑酮G得率为7.09 mg·g^-1,桑酮H得率为1.74 mg·g^-1,桑辛素得率为1.69 mg·g^-1,与预测值相比,相对误差分别为0.30%、0.41%、0.42%。结论:采用响应曲面法优化得到同时提取桑白皮中桑酮G、桑酮H、桑辛素的工艺科学合理,可用于扩大生产。
Objective:To establish a high performance liquid chromatography method for simultaneous determination of kuwanon G,kuwanon H and morusin in Mori Cortex and optimize the extraction technology of them by response surface methodology.Methods:The contents of kuwanon G,kuwanon H and morusin were used as the response values,the ethanol concentration,extraction temperature and extraction time were taken as the main factors.Based on the single factor experiment,the central combination design experiment was used to optimize the best extraction technology for the three ingredients.Results:The optimum condition for extracting kuwanon G,kuwanon H and morusin from Mori Cortex was as follows:ethanol concentration 68%,extraction temperature 74℃,extraction time 110 min,ratio of material to liquid 14∶1.The yield of kuwanon G was 7.09 mg·g^-1,the yield of kuwanon H was 1.74 mg·g^-1 and the yield of morusin was 1.69 mg·g^-1.Compared with the predicted values,the relative error was about 0.30%,0.41%,0.42%,respectively.Conclusion:The technology of extracting kuwanon G,kuwanon H and morusin from Mori Cortex obtained by response surface methodology is scientific and reasonable,and can be used to expand production.
作者
刘一涵
田云刚
王建霞
郭洪伟
魏华
LIU Yi-han;TIAN Yun-gang;WANG Jian-xia;GUO Hong-wei;WEI Hua(College of Biology and Environmental Science,Jishou University,Jishou 416000,China;Tujia Medicine Research Center in Hunan,Jishou 416000,China)
出处
《中国现代中药》
CAS
2020年第7期1100-1107,共8页
Modern Chinese Medicine
基金
国家中医药行业科研专项(201507002)
国家自然科学基金青年科学基金项目(81403088)
湖南省土家医药研究中心开放项目(20172001)
吉首大学校级科研项目(Jdy1858)。
作者简介
通信作者:魏华,副教授,研究方向:天然药物化学成分,E-mail:weihua20@126.com。
