摘要
针对新疆红枣,采用绿色、高效的低共熔溶剂(Deep Eutectic Solvent,DES)为提取剂,通过超声波辅助技术提取其中的功能性成分--环磷酸腺苷(cyclic adenosine 3',5'-monophosphate,cAMP)。研究低共熔溶剂的摩尔比、含水量以及料液比、超声时间、超声温度与cAMP提取量的关系,通过单因素实验和响应面优化试验,得出新疆红枣中cAMP提取的最佳条件为:氯化胆碱与丙三醇摩尔比为1:3,DES体系含水量为44%,红枣粉末与DES的料液比为1:35 g/mL,超声时间为45 min,超声温度为45℃,此时与同等超声条件下的水提法和醇提法相比,低共熔溶剂法提取cAMP的含量最高为(284.15±0.06)μg/g。因此,选用超声波辅助低共熔溶剂提取新疆红枣中的cAMP是获得较高提取量的一种新型、高效和安全的方法。
Using green and highly efficient deep eutectic solvent(DES)as the extraction agent,this study extracted the functional ingredient—cyclic adenosine 3',5'-monophosphate(cAMP)from Xinjiang jujube through ultrasound-assisted technology.The relationships between the molar ratio of deep eutectic solvent,water content of deep eutectic solvent,solid-liquid ratio,ultrasonic time,ultrasonic temperature and extraction amount of cAMP were studied.Through single factor experimentas and response surface optimization experiment,the optimal conditions for cAMP extraction from Xinjiang jujube were as follows:The molar ratio of choline chloride to glycerol was 1:3,the water content of DES system was 44%,the solid-liquid ratio of jujube powder to DES was 1:35 g/mL,the ultrasonic time was 45 min,and the ultrasonic temperature was 45℃.At this time,compared with water extraction and alcohol extraction under the same ultrasonic conditions,the highest content of cAMP extracted by deep eutectic solvent was(284.15±0.06)μg/g.Therefore,ultrasonic-assisted deep eutectic solvent extraction of cAMP in Xinjiang jujube was a new,efficient and safe method to obtain a higher extraction amount.
作者
张雪
刘娅
刘秀敏
单春会
ZHANG Xue;LIU Ya;LIU Xiumin;SHAN Chunhui(College of Food Science,Shihezi University,Shihezi 832000,China;Engineering Research Center of Xinjiang Ministry of Education for Fruit and Vegetable Storage and Processing,Shihezi 832000,China)
出处
《食品工业科技》
CAS
北大核心
2022年第14期243-250,共8页
Science and Technology of Food Industry
基金
新疆生产建设兵团重点领域创新团队建设计划项目(2017CB012)
新疆生产建设兵团南疆重点产业创新发展支撑计划项目(2018DB002)。
关键词
新疆红枣
环磷酸腺苷
低共熔溶剂
超声波辅助
高效液相色谱法
Xinjiang jujube
cyclic adenosine monophosphate
deep eutectic solvent
ultrasonic assisted
high performance liquid chromatography
作者简介
张雪(1997-),女,硕士研究生,研究方向:食品化学与营养学,E-mail:1103020650@qq.com;通信作者:单春会(1978-),男,博士,教授,研究方向:果蔬加工与贮藏,E-mail:972338194@qq.com。
