摘要
以陕西特产大枣为原料,研究了其药用成分多糖的提取和分离工艺.该工艺以蒸馏水为提取剂,所得多糖提取液经一定体积分数的乙醇沉淀、氯仿-正丁醇脱蛋白、双氧水脱色等工艺后,得到大枣多糖;在此基础上,分别采用单因素试验和正交试验对大枣多糖的醇沉工艺进行了优选.试验得出最佳醇沉工艺条件为:加入无水乙醇,使乙醇最终体积分数为70%,室温静置5h.该工艺提取所得大枣多糖提取率达到3.86%,经硫酸-苯酚法分析测定其多糖质量分数为41%.
The technology of extraction and separation of polysaccharide from zizyphus jujube was studied. Based on this study a mature extraction process of polysaccharide was put forward, And the optimum deposited conditions were obtained by orthogonal test： the polysaqccharide was deposited with pure ethanol （the content of ethanol was up to 70% ） for 5h. With this technology the yield ratio of polysaccharide was up to 3.86% and the content of polysaccharide was up to 41%.
出处
《宁夏工程技术》
CAS
2005年第3期265-267,共3页
Ningxia Engineering Technology
基金
陕西省科技发展计划基金资助项目(2000K05G3)
西安市科技攻关基金资助项目(GG200147)
关键词
大枣
多糖
水提
醇沉
jujube
polysaccharide
extraction
isolation
作者简介
李宏燕（1976-），女，硕士研究生，主要从事天然产物有效成分的提取分离研究。
