摘要
文中提出了一种基于双极电化学法制备图案化海藻酸钙水凝胶支架的方法,利用光刻技术在氧化铟锡玻璃上制造出多形状的电极,成功实现了海藻酸钙水凝胶在目标区域上的沉积。建立了水凝胶的高度生长模型,研究了反应时间、直流电源电压、驱动电极间距对所制备水凝胶高度的影响。结果表明,水凝胶的模型预测高度与实测值的误差在5%以内,且在反应时间为100 s、直流电源电压为12.5 V、驱动电极间距为30 mm时,制备的水凝胶密度较为均匀。最后,将细胞包埋于水凝胶20 h后,细胞存活率保持在80%以上。证明该方法制备的水凝胶支架对细胞损害小,对组织工程学体外生物支架构建具有较大的参考价值。
A method for preparing patterned calcium alginate hydrogel scaffold based on bipolar electrochemis-try was proposed.By photolithography technology,a multi shape electrode on indium tin oxide(ITO)glass was fabricated,which leads to the specific-area deposition of calcium alginate.Meanwhile,a height growth model was established,and the effects of three parameters,such as reaction time,DC power supply voltage and driving elec-trode spacing,on the height of the prepared hydrogel were studied.The results show that the error between the pre-dicted height of the hydrogel model and the measured value is less than 5%.When the reaction time is 100 s,the DC power supply voltage is 12.5 V,and the driving electrode spacing is 30 mm,the hydrogel density is uniform.Finally,the cells were embedded in hydrogel for 20 h,and the cell viability is maintained at over 80%.It is proved that the hydrogel scaffold prepared by this method has little damage to cells,and has great reference value for the construction of biological scaffold in vitro for tissue engineering.
作者
李长悦
解飞
马立
Changyue Li;Fei Xie;Li Ma(School of Mechanical Engineering and Automation,Shanghai University,Shanghai 200444,China)
出处
《高分子材料科学与工程》
EI
CAS
CSCD
北大核心
2022年第1期159-167,共9页
Polymer Materials Science & Engineering
基金
国家重点研发计划(2018YFB1309200)。
关键词
双极电化学法
海藻酸钙水凝胶
高度生长模型
组织工程
bipolar electrochemistry
calcium alginate hydrogel
height growth model
tissue engineering
作者简介
通讯联系人:马立,主要从事微操作机器人技术研究,E-mail:malian@shu.edu.cn。
